天然产物研究与开发2017，Vol.29Issue(3)：497-502,6.DOI:10.16333/j.1001-6880.2017.03.024
星点设计-效应面法优化莪术油中吉马酮的提取工艺
Optimization of Extraction Process of Germacrone from Zedoary Turmeric Oil by Central Composite Design and Response Surface Methodology
摘要
Abstract
Zedoary turmeric oil from crude extract of Rhizoma Curcuma has been used as an anti-cancer agent in Traditional Chinese Medicine.Germacrone is a natural compound isolated from zedoary turmeric oil.In this study,we optimized the extraction process of germacrone from zedoary turmeric oil by central composite design and response surface methodology.The process was carried out with the content of germacrone as dependent variable,soaking time,extraction time and the amount of water as independent variables which was based on single factor tests,and each level of independent variables was made by multiple linear regression and binomial fitting.Response surface methodology was used to optimized extraction process conditions and carry on predictive analysis.The HPLC method was used to test the determination of germacrone.The results showed that average content of germacrone was 8.13％ with a small deviation compared with the predicted value when optimized extraction conditions were applied with soaking time of 2.36 h,extraction time of 8.19 h and 9.98 times water.This optimized extraction process was simple and reasonable with a good predictability and can provide reference for extraction of germacrone.关键词
莪术油/吉马酮/星点设计-效应面法/提取工艺/含量测定Key words
zedoary turmeric oil/germacrone/response surface methodology/extraction technology/content determination分类
医药卫生引用本文复制引用
吴杰,冯钰,陈伟强,杨梦蝶,古金华,叶连宝..星点设计-效应面法优化莪术油中吉马酮的提取工艺[J].天然产物研究与开发,2017,29(3):497-502,6.基金项目
广东省自然科学基金(2015A030313586) （2015A030313586）
广东省科技计划(2015-110) （2015-110）
