武警医学2017，Vol.28Issue(2)：178-181,4.
液相色谱-串联质谱法测定红花中非法染色物新品红、金胺O和苏丹红Ⅳ含量的研究
Simultaneous determination of illegal dyes of New fuchsin, Auramine 0 and Sudan Ⅳ in Carthami Flos by LC-MS/MS
李婷婷 1刘莉 1曾桢 1孔卫东 1李良 2刘芬 3刘文剑2
作者信息
- 1. 610045,成都市食品药品检验研究院中药室
- 2. 330006南昌,武警江西总队医院医务处
- 3. 330006,南昌大学药学院
- 折叠
摘要
Abstract
Objective To develop an LC-MS/MS method for simultaneous determination of illegal dyes of New fuchsin,Auramine O and Sudan Ⅳ in Carthami Flos.Methods Using Agilent EC-C18 (2.1 mm × 50 mm,1.8 μm)column,the mobile phase consisted of 20 mmol/L ammonium formate + 0.1％ methane acid-acetonitrile using gradient elution.The column temperature was 40 ℃,ionization mode was ESI,and detection mode was MRM.The ion pair selected was:①New fuchsin m/z 330.3→223.2,m/z 330.3→300.2;②Auramine O m/z 268.2→147.2,m/z 268.2→252.2;③Sudan Ⅳ rn/z 381.2→224.1,m/z 381.2→91.1.Results The linear range of New fuchsin,Auramine O and Sudan Ⅳ was 0.1086-271.50 ng/ml,0.1392-348.00 ng/ml and 0.1352-338.00 ng/ml,respectively,the recoveries were 97.45％,96.44％ and 97.58％,respectively,and RSD was 0.70％,1.12％ and 0.89％,respectively.RSD of precision,stability and repeatability was less than 3％.Conclusions This method of extraction is simple to use,accurate for determination and highly sensitive.This method can be applied to the determination of illegal dyes of New fuchsin,Auramine O and Sudan Ⅳ in Carthami Flos.关键词
LC-MS/MS/红花/新品红/金胺O/苏丹红ⅣKey words
LC-MS/MS/Carthami Flos/New fuchsin/Auramine O/Sudan Ⅳ分类
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李婷婷,刘莉,曾桢,孔卫东,李良,刘芬,刘文剑..液相色谱-串联质谱法测定红花中非法染色物新品红、金胺O和苏丹红Ⅳ含量的研究[J].武警医学,2017,28(2):178-181,4.
