新疆农业科学2017，Vol.54Issue(3)：429-433,5.DOI:10.6048/j.issn.1001-4330.2017.03.006
籽瓜花粉活力测定最适培养条件研究
Study on the Optimal Cultural Conditions for Pollen Viability of Seed-Watermelon
摘要
Abstract
[Objective] Through the culture of seed-watermelon pollen,the effects of different concentrations of sucrose and boric acid as basic liquid medium at different culture temperatures and in different culture time on pollen germination in vitro are studied.[Method]In this project,the optimum culture medium was used to carry out in vitro culture at 6 temperature levels and at 4 time levels.[Result]When the culture temperature was 25-30,the highest pollen viability was obtained,and the optimal medium formula was: 15％sucrose,0.01％boric acid and 1％agar.[Conclusion]With the increased temperature,the germinal rates of pollen and the length of pollen tube were increased;when the temperature reached up to 30℃,the pollen germination rates and pollen length were both decreased.In addition,the germination rate and pollen length were increased by being cultured for 4 hours,and then the length was no longer increased after being cultured 6 hours at room temperature.关键词
籽瓜/花粉活力/培养基Key words
seed-watermelon/pollen viability/culture medium分类
农业科技引用本文复制引用
林明,李春燕,白晓山,潘竟海,陈友强,刘华君,邓超宏,王娟,李承业..籽瓜花粉活力测定最适培养条件研究[J].新疆农业科学,2017,54(3):429-433,5.基金项目
新疆维吾尔自治区科技支撑项目"不同生态区籽瓜标准化栽培技术研究与示范"(201531101) （201531101）
新疆维吾尔自治区青年科学基金项目"新疆籽瓜种质资源遗传多样性的ISSR分析"(2015211B014) （2015211B014）
新疆农业科学院青年科技基金项目"影响籽瓜花粉活力及结实率温度条件的研究"(xjnky-2014012)Autonomous Region Key Technology R&D Program"Research of The Standard Cultivation Technique of Seed-watermelon in Different Ecology Regions"(201531101) （xjnky-2014012）
Autonomous Region Youth Scientific Funds R&D Program"Analysis of Genetic Diversity of Germplasm Resources of Seed-watermelon Revealed by ISSR in Xinjiang"(2015211B014) （2015211B014）
Autonomous Region Academy of Agricultural Sciences'Youth Scientific Funds R&D Program"Study on the Temperature Conditions for Pollen Viability and Fruiting Rate of Seed-watermelon"(xjnky-2014012) （xjnky-2014012）
