畜牧兽医学报2017，Vol.48Issue(4)：595-604,10.DOI:10.11843/j.issn.0366-6964.2017.04.002
奶牛分子系谱构建或亲权鉴定的微卫星标记筛选
Selection of Microsatellite Markers Used for Pedigree Tree Construction and/or Paternity Testing in Chinese Holstein Cows
摘要
Abstract
How to use less and optimized genetic markers to achieve convenient and efficient parentage determination for cows was our research purpose,so as to effectively apply them into paternity test and pedigree construction.Eighteen microsatellite markers with high polymorphisms in cattle recommended on the web of ISAG were selected in this study,8 markers that could be efficiently amplified were genotyped in 300 Chinese Holstein cows from the Jiabao Farm,based on fluorescent primer and automatic sequencing techniques.The results of paternity testing efficiency showed that the average of allele number,polymorphism information content (PIC),observed heterozygosity (Ho),and expected heterozygosity (He) were 14.63,0.747,0.718 and 0.773,respectively.In 3 different conditions of known and/or unknown parent-offspring,their combined probabilities of exclusion (CPEs) were 0.990,0.999 and 0.999,respectively.The results of paternity testing from 8 father-son showed that the 8 markers could successively be used to identify correct parentage and eliminate error records in the pedigree.Combined probability of exclusion analysis showed that when the number of microsatellite markers increased to 4 (TGLA227,TGLA122,BMC1207,BM103),all CPEs under 3 different situations were 0.949 or higher,which met the requirements of paternity tests in individual case.While,the results of molecular pedigree tree building at population level revealed that when the number of microsatellite sites increased to 6 (TGLA227,TGLA122,BMC1207,BM103,INRA037 and INRA134),inbreeding coefficient and total inbreeding coefficient within population were significantly increased,suggested that the 6 markers could efficiently be applied for pedigree tree construction and/or paternity testing for Chinese Holstein cows population(＜200 individuals).关键词
中国荷斯坦牛/微卫星位点/排除概率/亲子鉴定Key words
Chinese Holstein cows/microsatellite markers/probability of exclusion (PE)/paternity testing分类
农业科技引用本文复制引用
殷彬,岳书俭,俞英,曹巧巧,王中华,师科荣..奶牛分子系谱构建或亲权鉴定的微卫星标记筛选[J].畜牧兽医学报,2017,48(4):595-604,10.基金项目
国家自然科学基金(31402054) （31402054）
山东省自然科学基金(ZR2013CM013) （ZR2013CM013）
山东省农业良种工程项目(2016LZGC030) （2016LZGC030）
国家现代奶业产业技术体系(CARS-37) （CARS-37）
山东省泰山学者种业创新人才团队计划培养项目(2014LZ07-04) （2014LZ07-04）
