畜牧兽医学报2017，Vol.48Issue(4)：605-617,13.DOI:10.11843/j.issn.0366-6964.2017.04.003
麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析
Identification of SNPs in GH, GHR and GHSR Genes and Their Association with Body Measurement Traits in Maiwa Yak
摘要
Abstract
The aims of this study were to determine the genetic diversity of the GH,GHR and GHSR genes in Maiwa yak,to reveal the association between different genotypes and growth traits,to provide the theoretical foundation for the expression regulation of the candidate gene in yak and to look for the molecular marker that can be used for the assistant selection for genetic breeding.The DNA pool technology,PCR-RFLP and the direct sequencing were adopted to study the genetic polymorphism of the GH,GHR and GHSR genes in Maiwa yak and to analyze the association between the polymorphism of the candidate genes and the growth traits,such as withers height,body length,chest circumference,cannon circumference and body weight.The results showed that:1)The GH,GHR and GHSR genes of the Maiwa yak were all with the polymorphism,among which the GH gene had 2 SNP sites(A757G and T949C).Three mutation sites are found on the GHR gene(T2416C,T3490C and A7500G).T1387C and T3006C mutations existed on the GHSR gene.2)The fitness test indicated that the A7500G of the GHR gene in the pink lip group and the T3006C of the GHSR gene in the pure black group all deviated from the Hardy-Weinberg equilibrium while the other sites all conformed to the Hardy-Weinberg equilibrium.3)The different significance test indicated that the T2416C,T3490C and A7500G of the GHR gene were significantly related to the cannon circumference of the Maiwa yak (P＜0.01),while the T1387C of the GHSR gene was significantly related to body weight (P＜0.05).The results indicate that the GH,GHR and GHSR genes of the Maiwa yak are all with the genetic polymorphism,and it is deduced that the T2416C,T3490C and A7500G of the GHR gene as well as the T1387C of the GHSR gene may be the major effect genes influencing the cannon circumference and body weight of the Maiwa yak or the loci may be closely linked to the major effect genes and they can be regarded as the genetic markers for the assistant selection.关键词
麦洼牦牛/GH基因/GHR基因/GHSR基因/多态性/相关分析Key words
Maiwa yak/GH gene/GHR gene/GHSR gene/polymorphism/association analysis分类
农业科技引用本文复制引用
海汀,柴志欣,钟金城..麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析[J].畜牧兽医学报,2017,48(4):605-617,13.基金项目
四川省科技计划项目(2015JY0248) （2015JY0248）
中央高校基本科研项目(2016NZYQN30) （2016NZYQN30）
国家肉牛牦牛产业技术体系(2017) （2017）
