畜牧兽医学报2017，Vol.48Issue(4)：618-626,9.DOI:10.11843/j.issn.0366-6964.2017.04.004
基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选
Differentially Expressed Proteins Screening in Different Parts of Sika Deer Velvet Antler by Comparative Proteomics
摘要
Abstract
To screen the differentially expressed proteins in different parts of sika deer velvet antler,the parts of sika deer velvet antlers of Lapian,Xuepian and Gupian were selected as experimental material.The differentially expressed proteins were identified by two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF MS,and analyzed using bioinformatics methods.The result showed that 37 differentially expressed proteins were detected,which were involved in the biological process of multicellular organismal process,detoxification,cellular component organization or biogenesis,cell aggregation,localization,response to stimulus,biological adhesion,developmental process,single-organism process and immune system.There were 18,5 and 14 up-regulated proteins in the Lapian,Xuepian,Gupian,respectively.We found that EFB1 and CRABP1 might play vital role in the process of velvet antler accelerated growth,and APOA1,FABP4,TTR might play vital role in the process of velvet antler ossification.Taken together,the result provide a basis for further research on the molecular mechanisms involved in the accelerated growth and ossification of deer velvet antler.关键词
梅花鹿/鹿茸/不同部位/双向凝胶电泳/蛋白质组学Key words
sika deer/velvet antler/different parts/two-dimensional gel electrophoresis/proteome分类
农业科技引用本文复制引用
刘华淼,鞠妍,张然然,邢秀梅..基于蛋白质组学技术的鹿茸不同部位差异蛋白筛选[J].畜牧兽医学报,2017,48(4):618-626,9.基金项目
特种动物遗传资源创新团队(CAAS-ASTIP-201X-ISAPS) （CAAS-ASTIP-201X-ISAPS）
