畜牧兽医学报2017，Vol.48Issue(4)：627-636,10.DOI:10.11843/j.issn.0366-6964.2017.04.005
营养诱导舍饲哈萨克羊非繁殖季节卵巢组织中miRNAs差异表达分析
Analyzing the Differential Expression of miRNAs in Ovary of Stall-feed Kazakh Sheep Induced by Nutrition in Non-breeding Season
摘要
Abstract
To further explore the theoretical basis of the microRNAs in regulating the non-breeding season estrous in sheep,characterization of ovarian microRNAs were constructed and analyzed in estrous and anestrous of non-breeding season induced by nutrition by Solexa sequencing.In this study,the Kazakh sheep with different nutrition levels were used as the research object.In non-breeding season,3 estrous (EN) and 3 anestrous (AN) sheep were slaughtered with their ovary tissue(O) collected,respectively.Two miRNA libraries were constructed and analyzed by the bioinformatics methods.The real-time quantitative PCR (qRT-PCR) was used for validating the differentially expressed miRNAs betweent the 2 periods in ovary.The results showed that the clean reads of 8 665 978 (OEN) and 9 071 102 (OAN) were obtained in the two libraries,and most of the small RNA sequences were between 21 and 23 nt.Compared the two ovary (OEN vs OAN) libaries,the 9 known and 104 novel miRNAs had significant expression difference (P＜0.01).The expression level of 6 randomly selected miRNAs by qRT-PCR was the same as the result by Solexa sequencing.KEGG pathway analysis showed that these target genes of difference miRNAs were primarily involved in nutrition regulatory related pathways,such as glycine,serine and threonine metabolism,amino sugar and nucleotide sugar metabolism,D-arginine and D-ornithine metabolism,glycerol phospholipid metabolism,and so on.The differentially expressed miRNAs may play an important role in non-breeding season estrous of sheep through nutrition metabolism pathway by the analysis of predicted target and enriched pathway analysis.关键词
哈萨克羊/发情期/乏情期/卵巢/Solexa测序/microRNAKey words
Kazakh sheep/estrus/anestrus/ovary/solexa sequencing/microRNA分类
农业科技引用本文复制引用
雷小萍,林杉,于要升,梁慧慧,赵宗胜..营养诱导舍饲哈萨克羊非繁殖季节卵巢组织中miRNAs差异表达分析[J].畜牧兽医学报,2017,48(4):627-636,10.基金项目
国家自然科学基金项目(31260537) （31260537）
