畜牧兽医学报2017，Vol.48Issue(4)：645-651,7.DOI:10.11843/j.issn.0366-6964.2017.04.007
稀释和低温保存对鸡精液品质和受精能力的影响
The Effects of Dilution and Hypothermic Preservation on Chicken Semen Quality and Fertility
摘要
Abstract
This study explored the influence of dilution and low-temperature preservation on chicken semen quality and fertility,aiming at optimizing the short-term preservation conditions for the chicken semen.The semen samples collected from 30 Beijing-You chickens were mixed gently and divided into 4 groups:Group A:original semen as a control group;Group B:diluted with BPSE (1 ∶ 1);Group C:diluted with BPSE (1∶1) after removing seminal plasma;Group D:further diluted with BPSE (1:1)based on group C.Semen of each group was further divided into two proportions.One was used for the semen quality estimation and artificial insemination (AI) and the other was stored at 4 ℃ for semen quality evaluation at 8,16,and 24 hours post storage and also AI at 24 hours post storage.For each group,around 120 eggs were collected for hatching to evaluate the fertility and hatching performance.As a result of different dilution groups,sperm motility of group C and D were lower than that of group A and B.Group A,B,and C did not show any difference in fertility or hatchability.The group D,however,showed significantly lower fertility and hatchability (P＜0.01).The results of 4 ℃ storage revealed that,sperm motility of 4 groups decreased significantly (P＜0.01).Group C and D decreased more quickly than group A and B.The sperm deformity were also increased during the storage (P＜0.05),which were mainly characterized by swelling heads,bending necks,and cracking tails.Fertility and hatchability of group A,B,and C decreased significantly after 24 hours of storage at 4 ℃ (P＜0.05).The fertility and hatchability of group D after storage were 71 ％ and 82％,respectively,which were as high as those of before storage.The results in the present study indicated that,firstly,dilution of semen is feasible to increase the AI efficiency without decreasing the fertility;secondly,removing semen plasma and diluting before 4 ℃ storage may maintain the sperm motility and guarantee higher fertility.关键词
鸡/精液/低温保存/BPSE/稀释液/受精率Key words
chicken/semen/hypothermic preservation/BPSE/dilution/fertility分类
农业科技引用本文复制引用
徐松山,孙研研,李云雷,薛夫光,刘一帆,许红,陈继兰..稀释和低温保存对鸡精液品质和受精能力的影响[J].畜牧兽医学报,2017,48(4):645-651,7.基金项目
现代农业产业技术体系(CARS-41) （CARS-41）
家养动物种质资源平台(2016) （2016）
农业部“948计划”项目“高产蛋鸡纯系及鸡冻精保种技术引进和利用”(2015-28) （2015-28）
