畜牧兽医学报2017，Vol.48Issue(4)：731-739,9.DOI:10.11843/j.issn.0366-6964.2017.04.017
刚地弓形虫钙依赖蛋白激酶9对小鼠Ana-1巨噬细胞功能的影响
Effects of Calcium Dependent Protein Kinase 9 of Toxoplasma gondii on the Functions of Mouse Ana-1 Macrophages in vitro
摘要
Abstract
This study aimed to investigate regulation of calcium dependent protein kinase 9 of Toxoplasma gondii to the macrophages of mouse.The laser confocal microscopy was utilized to check whether CDPK9 was able to bind to Ana-1 macrophages.After co-incubation with CDPK9,the proliferation of macrophages was checked by cell counting kit-8 (CCK-8).Then apoptosis and phagocytosis of macrophages were calculated by flow cytometry.NO production of cells was evaluated by Total Nitric Oxide Assay kit and expressions of TNF-α,TGF-β1,IL-10 and IL-1β were detected by ELISA research reagent.The pictures of laser confocal microscopy showed that CDPK9 could bind to macrophages.After co-incubation with CDPK9,the proliferation of macrophages was suppressed.Moreover,both apoptosis and phagocytosis rates of cells were increased.However,NO production was up-regulated by CDPK9.Concerning cytokines,production of IL-1β and TNF-α was increased,while the production of TGF-β1 and IL 10 was decreased.Our study demonstrated that CDPK9 was able to bind to macrophages and exert its effects on the activity and functions of Ana-1 macrophages via different pathways.关键词
刚地弓形虫/钙依赖蛋白激酶9/巨噬细胞/流式细胞术/细胞因子Key words
Toxoplasma gondii/calcium dependent protein kinase 9 (CDPK9)/macrophage/flow cytometry/cytokine分类
农业科技引用本文复制引用
马群山,刘欣超,孙晓妮,王帅,徐立新,宋小凯,严若峰,李祥瑞..刚地弓形虫钙依赖蛋白激酶9对小鼠Ana-1巨噬细胞功能的影响[J].畜牧兽医学报,2017,48(4):731-739,9.基金项目
农业部公益性行业专项(200903036) （200903036）
