畜牧兽医学报2017，Vol.48Issue(4)：761-768,8.DOI:10.11843/j.issn.0366-6964.2017.04.020
玉米赤霉烯酮降解菌的筛选及其活性检测
Screening and Activity Detection of a Bacterial Strain Degrading Zearalenone
摘要
Abstract
Zearalenone(ZEN) is one of the main mycotoxin endangering animal health.The aim of this research was to screen bacteria with the ability to degrade ZEN efficiently,and detect their degradation characteristics and toxicity.ZEN was used as the sole carbon source for the preliminary screening.And then the strains fermentation broth degradation rate was as the index for the second selection.The 16S rRNA sequence analysis was used to identify the strain with the highest ZEN degradation rate.The ZEN degradation characteristics of the obtained strain was studied,and the activity of the ZEN degradation product was tested in MCF-7 cells.Forty strains were screened out in the preliminary experiment,and strain H6 with the highest ZEN degradation rate was selected after the secondary screening.Strain H6 was identified as the Bacillus amyloliquefaciens by 16S rRNA sequence analysis.At 37 ℃,180 r · min-1,72 h,the degradation rates of strain H6 fermentation liquor to 1 and 20 μg · mL-1 ZEN were 95.6％ and 85.8％,respectively.Among that,the degradation rates of the culture supernatant and cells to 1 μg · mL-1 ZEN were 68.93％ and 28.70％,respectively.The ZEN degradation activity of strain H6 culture supernatant was significantly decreased after treated with proteinase K,proteinase K combined with SDS and heating,respectively.Compared with the control group,the proliferation rate of MCF-7 cell was significantly reduced when treated with ZEN degradation product.In conclusion,strain H6 with the highest ZEN degradation rate was identified as Bacillus amyloliquefaciens by 16S rRNA sequence analysis.The extracellular enzymes secreted by strain H6 could degrade ZEN,and significantly reduce the estrogen activity.关键词
真菌毒素/玉米赤霉烯酮/解淀粉芽孢杆菌/生物降解Key words
Mycotoxin/Zearalenone/Bacillus amyloliquefaciens/biodegradation分类
农业科技引用本文复制引用
魏单平,刘玉洁,孙向丽,闫峰宾,蒋瑞瑞,康相涛,王彦彬..玉米赤霉烯酮降解菌的筛选及其活性检测[J].畜牧兽医学报,2017,48(4):761-768,8.基金项目
河南省重点科技公关项目(152102110058) （152102110058）
河南省高等学校重点科研项目(16A230011) （16A230011）
教育部创新团队发展计划(IRT16R23) （IRT16R23）
