烟草科技2017，Vol.50Issue(3)：1-7,7.DOI:10.16135/j.issn1002-0861.2016.0388
硅对铅胁迫下烟草铅含量、形态和亚细胞分布的影响
Effects of silicon on content, morphology and subcellular distribution of lead in tobacco under lead stress
摘要
Abstract
To study the effect of silicon on tobacco growth under lead stress and the underlying mechanism, hydroponic experiments were conducted on K326 with exogenous lead in the form of lead nitrate [Pb(NO3)2] and exogenous silicon in the form of potassium silicate (K2SiO3). KNO3 and HNO3 were used to control the effects of increased pH and K+ of nutrient solutions caused by K2SiO3. In total,five treatments were set as following: control(CK),100 μmol·L-1 Pb2+(Pb100),300 μmol·L-1 Pb2+(Pb300),100 μmol·L-1 Pb2++1.0 mmol·L-1 SiO2(Pb100+Si)and 300 μmol·L-1 Pb2++1.0 mmol·L-1 SiO2(Pb300+Si). The results showed that comparing with the control,Pb100 and Pb300 treatments decreased the plant height and biomass of tobacco by 51.44%,69.92% and 51.43%,64.55%,respectively. Exogenous silicon could alleviate the harmfulness of lead stress on tobacco. Comparing with Pb100 and Pb300 treatments,the plant height and biomass of tobacco under Pb100+Si and Pb300+Si treatments increased by 69.23%,35.99% and 84.94%,76.75%,respectively; the differences were statistically significant. Silicon inhibited the migration of lead from root upwards and reduced the lead contents in stalk, lower leaves and upper leaves. Silicon decreased the proportion of ethanol-extractable lead in root and NaCl-extractable lead in leaf,but increased the proportion of HCl-extractable and residual lead both in root and leaf. Comparing with Pb100 and Pb300 treatments,the lead proportion in nuclei of roots under Pb100+Si and Pb300+Si treatments decreased by 25.29% and 5.06%,respectively;the lead proportion in chloroplasts and mitochondria leaf decreased by 29.22%,54.95% and 38.09%,51.46%,respectively.关键词
烟草/硅/铅胁迫/铅含量/铅形态/亚细胞分布Key words
Tobacco/Silicon/Lead stress/Lead content/Morphology of lead/Subcellular distribution分类
农业科技引用本文复制引用
李荟星,徐宋萍,郑明瑜,罗丽娟,唐莉娜,陈星峰,李延..硅对铅胁迫下烟草铅含量、形态和亚细胞分布的影响[J].烟草科技,2017,50(3):1-7,7.基金项目
中国烟草总公司福建省公司科研项目"硅对烤烟产量品质的影响及其施用技术研究"(闽烟合同[2013]63 号) （闽烟合同[2013]63 号）
福建农林大学科技创新专项基金项目"农业废弃物对土壤重金属污染的修复作用"(105/KF2015072). （105/KF2015072）
