烟草科技2017，Vol.50Issue(3)：47-51,5.DOI:10.16135/j.issn1002-0861.2016.0327
烟籽油对高胆固醇血症大鼠胆固醇代谢的影响
Effects of tobacco seed oil on metabolism of cholesterol in rats with hypercholesterolemia
摘要
Abstract
In order to investigate the effects of tobacco seed oil on serum cholesterol level and expressions of regulatory genes related to cholesterol metabolism in livers of rats,the levels of total cholesterol (TC), triacylglycerol (TG),high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in serum were determined by using rats fed with a high level cholesterol diet as animal models. The rats were randomly divided into control groups (blank control group,model control group,fish oil control group) and experimental groups (three groups fed with tobacco seed oil at low,middle and high doses, separately). The expressions of regulatory genes related to cholesterol metabolism in livers of rats were measured with real-time PCR method. Liver tissues were sliced and stained to observe the steatosis of hepatic cells of rats. The results showed that:1) Tobacco seed oil effectively reduced the levels of TC and LDL-C in serum and atherogenic index of rats,improved hepatic steatosis. 2) Tobacco seed oil down-regulated the expression of HMGCR gene and up-regulated the expression of LDLR gene in livers of rats with hypercholesterolemia. Therefore,tobacco seed oil has a significant effect of lowering total cholesterol,the mechanism might be actioned by regulating the expressions of genes related to cholesterol metabolism in livers.关键词
烟籽油/高胆固醇血症/胆固醇/代谢Key words
Tobacco seed oil/Hypercholesterolemia/Cholesterol/Metabolism分类
医药卫生引用本文复制引用
侯小东,梁惠,张忠锋,张怀宝,毛新新,傅泳,付秋娟,杜咏梅..烟籽油对高胆固醇血症大鼠胆固醇代谢的影响[J].烟草科技,2017,50(3):47-51,5.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC05). （ASTIP-TRIC05）
