烟草科技2017，Vol.50Issue(3)：57-64,8.DOI:10.16135/j.issn1002-0861.2016.0361
响应面法优化漆酶降解梗丝木质素的工艺
Optimization of lignin degradation by laccase in cut stem with response surface method
摘要
Abstract
In order to reduce the lignin content in cut stems,to improve the quality of cut stems and to increase their proportion in cigarette blends,single factor experiments were conducted to study the effects of four factors involved in laccase treatment of cut stems(treatment temperature,treatment time,addition rate of laccase,pH of laccase solution) on the degradation of lignin in cut stems. The degradation technology was optimized by response surface method,the cut stems were analyzed by scanning electron microscope before and after the laccase treatment,and the sensory quality of cigarettes made of the treated and untreated cut stems was panel tested,separately. The results showed that:1) The optimized treatment condition was at temperature 45.0 ℃, treatment time 3.0 h,the addition rate of laccase 20.0 U/g,and pH of laccase solution at 6.0. The calculated degradation rate of lignin was 56.68%,and the measured value was 55.05%. 2)The surface of the treated cut stems by laccase was smoother with less burrs. 3) Comparing with the control,the aroma quality,aroma quantity and aftertaste of the test cigarette smoke were improved,the levels of offensive odor and irritation were reduced,while the strength,combustibility and ash color did not change obviously. In summary,the optimized treatment condition for degrading lignin in cut stems with laccase is reliable,which provides the potential to expand the application of cut stem in cigarette.关键词
梗丝/木质素/降解率/响应面法/漆酶/卷烟/感官评价Key words
Cut stem/Lignin/Degradation rate/Response surface method/Laccase/Cigarette/Sensory evaluation分类
轻工纺织引用本文复制引用
于建军,魏登辉,田斌强,常安然,张耸,刘小勇,赵士举..响应面法优化漆酶降解梗丝木质素的工艺[J].烟草科技,2017,50(3):57-64,8.基金项目
河南省高等学校重点科研项目"浓香型特色烟叶规模开发与工业利用研究"(17A210018) （17A210018）
中国烟草总公司特色优质烟叶开发重大专项"烤烟烟叶淀粉结构与特性研究"(Ts-01-2011007) （Ts-01-2011007）
河南农业大学重点学科基金项目"微波辐射绿色化提取木质素及制取纤维素地膜"(30600980). （30600980）
