微创医学2017，Vol.12Issue(2)：170-174,5.DOI:10.11864/j.issn.1673.2017.02.04
抗巨噬细胞移动抑制因子单抗对结肠炎小鼠结肠NF-κB、IκB表达的影响
Effect of anti-macrophage migration inhibitory factor on NF-κB、IκB Expression in mice with ulcerative colitis
摘要
Abstract
Objective To investigate the effect of anti-macrophage migration inhibitory factor monoclonal antibody on NF-κB p65、IκBα expression in mice with ulcerative colitis.Methods Acute ulcerative colitis was induced by 5% dextran sulfate sodium(DSS).Then the protective effect of anti-MIF Ab on ulcerative colotis in mice was observed.Forty mice were randomly distributed into normal control group, UC model group, anti-MIF Ab intervention group and saline group.During modeling and the period of administration, to observe score Disease Activity and general situations Index(DAI) of mice, after 8 days killing all mice,performing the gross damage score in colon, observing the colonic histopathological changes under the light microscope on HE staining.The expression of NF-κB p65、IκBα protein was detected by immunohistochemical staining.Results Compared with normal control mice, the level of NF-κB p65 protein increased significantly in colon tissue of mice with UC,the level of IκBα slightly increased.After treatment with anti-MIF Ab , the expression level of NF-κB p65 significantly lower than that in UC model group,but the expression level of IκBα increased.Conclusions These findings suggest that anti-MIF Ab had a protective effect on DSS-induced ulcerative colitis in mice down-regulating NF-κB p65 protein expression.Thus to inhibit the NF-κB signaling pathway, excessive activation in the colon tissue of UC mice.关键词
抗巨噬细胞移动抑制因子单抗/葡聚糖硫酸钠(DSS)/溃疡性结肠炎(UC)/核因子-κB(NF-κB)/NF-κB抑制蛋白(IκB)Key words
Macrophage migration inhibitory factor monoclonal antibody(anti-MIF Ab)/Dextran sulfate sodium(DSS)/Ulcerative colitis(UC)/Nuclear factor-κB (NF-κB)/Inhibitor of NF-κB(IκB)分类
医药卫生引用本文复制引用
唐光耀,张启芳,王柏涛,唐澄海,孟云超,李西融,张海莲,张晶..抗巨噬细胞移动抑制因子单抗对结肠炎小鼠结肠NF-κB、IκB表达的影响[J].微创医学,2017,12(2):170-174,5.基金项目
广西桂林市科学研究与技术开发计划项目(编号:科技攻关20140120-7-2) （编号:科技攻关20140120-7-2）
广西医疗卫生重点科研课题(编号:重2011018) （编号:重2011018）
