医药导报2017，Vol.36Issue(5)：484-488,5.DOI:10.3870/j.issn.1004-0781.2017.05.006
四季抗病毒合剂抗A16型柯萨奇病毒作用
Effect of Siji Kangbingdu Mixture on Inhibition of the Proliferation of Coxsackievirus A16
摘要
Abstract
Objective To verify the antiviral effects of Siji Kangbingdu mixture (SJKBDM) against coxsackievirus A16 (CoxA16).Methods Vero cells and 5-day-old suckling mice, injected with 75/50 and 1×106 TCID50 CoxA16, were used as evaluation models.The preventive influences of SJKBDM against CoxA16 in Vero cells were assessed in the models.The effects of SJKBDM on the mortality, survival time, change rate of body weight, and clinical symptom scores of suckling mice were observed.Results ①The half maximal inhibitory concentration of SJKBDM on Vero cells was 9.59 mg·mL-1.②Toxic effects were not observed from 32.3 g·kg-1 single dose or continuous intraperitoneal injectin of SJKBDM in suckling BALB/c mice.③The SJKBDM had significant inhibitory effect against CoxA16 virus.Doses higher than 1.22 mg·mL-1 could significantly improve the Vero cell survival rate, and the SJKBDM inhibition of 75/50 TCID50 CoxA16 induced pathological changes in Vero cells.④The SJKBDM significantly improved clinical symptoms of mice with CoxA16 viral infection, especially with crude drug doses of higher than 1.62 g·kg-1.The survival rate and other indicators were comparable or slightly higher compared with ribavirin, and the clinical score was higher than that of ribavirin.Conclusion The SJKBDM has significant inhibitory effect on CoxA16 cell proliferation, significantly decreases death rate, and improves clinical symptoms of mice infected with CoxA16 virus.关键词
四季抗病毒合剂/CoxA16病毒/Vero细胞/抗病毒作用Key words
Siji Kangbingdu mixture/Coxsackievirus A16/Vero cells/Antiviral effect分类
医药卫生引用本文复制引用
郑龙,肖会敏,郭伦锋,刘洋,顾丙权,赵荣,王四旺..四季抗病毒合剂抗A16型柯萨奇病毒作用[J].医药导报,2017,36(5):484-488,5.基金项目
陕西省科技统筹创新工程计划项目(2016-KTTSSF01-0-02) （2016-KTTSSF01-0-02）
