医药导报2017，Vol.36Issue(5)：532-537,6.DOI:10.3870/j.issn.1004-0781.2017.05.017
衍生化技术用于生物基质中类固醇类激素分析的研究进展
Progress of Chemical Derivatization in Analysis of Endogenous Anabolic Steroids in Bio-matrices
摘要
Abstract
The content of endogenous anabolic steroids is extremely low in biological matrices.Its chemical structure contains polar functional groups such as hydroxyl and carbonyl, which limits their applicability in GC-MS.The lack of ionized groups in the chemical structure leads to poor sensitivity in LC-MS, which plays a significant role in various physiological activities analysis.It is an effective way to enhance the response of mass spectrometer by modifying the chemical structure of endogenous anabolic steroids through derivatization technology.This review summarizes various derivatization reagents and corresponding derivatization processes of endogenous anabolic steroids analysis in the methods based on different testing instruments and methodologies.The advantage and disadvantage of all kinds of derivatiaztion methods and the prospect of the endogenous anabolic steroids derivatiaztion techniques are also discussed.关键词
类固醇类激素/衍生化/仪器分析Key words
Endogenous anabolic steroids/Derivatization/Instrument analysis分类
医药卫生引用本文复制引用
胡秋菊,杨建云,肖炳坤,黄荣清..衍生化技术用于生物基质中类固醇类激素分析的研究进展[J].医药导报,2017,36(5):532-537,6.基金项目
十二五国家科技重大专项"重大新药创制"综合性新药研究技术大平台(2012ZX09301003-001-010) （2012ZX09301003-001-010）
