应用化学2017，Vol.34Issue(4)：379-384,6.DOI:10.11944/j.issn.1000-0518.2017.04.160302
球状功能性烟草花叶病毒纳米颗粒的制备及表征
Preparation and Characterization of Functional Tobacco Mosaic Virus Spherical Nanoparticles
摘要
Abstract
Tobacco mosaic virus(TMV) due to its good biocompatibility, monodisperse, multivalent, low cost and other advantages, has been used as the basic building blocks used in optoelectronic devices, tissue engineering, vaccine vector, inorganic material preparation fields.However, compared to the rod like TMV, spherical TMV nanoparticles with no nucleic acid molecules are more resistant to environmental impact and have a larger specific surface area.The genetic and chemical modification of TMV, such as cysteine mutants of TMV(TMV TMV-Cys), lysine mutant of TMV(TMV-EPMK) and β-cyclodextrin(β-CD) modified TMV(TMV-β-CD) were conducted by thermal denaturation, and we studied their formation process and functionality.The results shows that the modified TMV can transit into the uniform spherical nanoparticles, and the functional groups exposed to the surface of the nanoparticles still keep their reactive ability.关键词
烟草花叶病毒/热变性/球型纳米颗粒/功能性/突变体Key words
tobacco mosaic viruses/thermal denaturation/spherical nanoparticles/functionality/mutant分类
化学化工引用本文复制引用
吴业红,赵霞,胡君,林园,王倩..球状功能性烟草花叶病毒纳米颗粒的制备及表征[J].应用化学,2017,34(4):379-384,6.基金项目
国家自然科学基金(21374119,21429401)、长春应用化学研究所高分子物理与化学国家重点实验室基金以及天津大学精密测试技术及仪器国家重点实验室开放基金资助 （21374119,21429401）
Supported by the National Natural Science Foundation of China(No.21429401, No.21374119), the State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry and the State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University （No.21429401, No.21374119）
