应用生态学报2017，Vol.28Issue(4)：1168-1176,9.DOI:10.13287/j.1001-9332.201704.002
不同施肥模式对雷竹林土壤真菌群落特征的影响
Effects of different fertilization regimes on soil fungal communities under Phyllostachys violascens stand
摘要
Abstract
In order to investigate the effect of fertilization on soil fungal community of Phyllostachys violascens stand,we characterized the abundance and community structure of soil fungi using the methods of terminal restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR.The experimental treatments included blank control (CK),chemical fertilizer (CF),organic manure (M),mixture of chemical fertilizer and organic manure (CFM) and mixture of chemical fertilizer and organic manure plus mulching (CFMM).The results showed that fungal community structure and diversity were markedly affected by different fertilization regimes.In 0-20 cm soil layer,CF and CFMM treatments resulted in a significant reduction of Shannon and evenness indices.Soil fungi under CK,M,CFMM treatments in 0-20 cm soil layer and CK,CF,CFMM treatments in 20-40 cm soil layer formed independent community structures.Soil fungal abundance showed significantly positive correlation with the concentrations of soil total nitrogen,ammonium nitrogen and nitrate nitrogen.Organic matter,ammonium nitrogen,available phosphorus and available nitrogen concentrations were the main factors that influenced the variation of fungal community comnposition.The above findings indicated that 0-20 cm and 20-40 cm soil layers had different characteristics in response to different fertilization regimes under P.violascens stand.The soil fungal community in the 0-20 cm laver was mainly affected by soil organic matter,while that in the 20-40 cm layerwas sensitive to the input of chemical fertilizer.The effect of fertilization on the diversity of fungal community mainly occurred in the 0-20 cm soil layer.关键词
施肥模式/土壤真菌/雷竹林/群落特征/土层Key words
fertilization mode/soil fungi/Phyllostachys violascens stand/community characteristic/soil layer引用本文复制引用
陈志豪,梁雪,李永春,李永夫,徐秋芳,邬奇峰,李松昊,沈振明..不同施肥模式对雷竹林土壤真菌群落特征的影响[J].应用生态学报,2017,28(4):1168-1176,9.基金项目
本文由国家自然科学基金项目(31200473,31470626)、浙江省自然科学基金项目(LY15C160006,LY14C160007)和浙江省教育厅科研项目(Y201225759)资助 This work was supported by the National Natural Science Foundation of China (31200473,31470626),the Natural Science Foundation of Zhejiang Province,China (LY15C160006,LY14C160007) and the Science Foundation of Education Department of Zhejiang Province,China (Y201225759). （31200473,31470626）
