应用生态学报2017，Vol.28Issue(4)：1377-1386,10.DOI:10.13287/j.1001-9332.201704.006
基于COI和ND5序列的中国南部唇(鱼骨)和间(鱼骨)群体遗传结构
Genetic structure of Hemibarbus labeo and Hemibarbus medius in South China based on mtDNA COI and ND5 genes
摘要
Abstract
Both Hemibarbus labeo and H.medius (Cypriniformes:Cyprinidae:Gobioninae) are primary freshwater fishes and are widely distributed.As such,they provide an ideal model for phylogeographical studies.However,the similarity in morphological characters between these two species made the description of their distributions and the validation of species quite challenging.Here we employed variations in the DNA sequences of mitochondrial COI and ND5 genes (2151 bp) to solve this challenge and to study the population genetics structure of these two species.Among the 130 specimens belonging to 8 populations of H.labeo and 9 populations of H.medius from 17 drainage systems in southern China,196 variable sites (9.1％ in the full sequences) falling into 50 haplotypes were identified.The haplotype diversity (h) and the nucleotide diversity (π) were 0.964 and 0.019,respectively,indicating a high level of genetic diversity and an evolutionary potential in both species.The result of neighbor-joining tree based on composite nucleotide sequences of the mtDNA COI and ND5 genes showed that the H.labeo and H.medius fell into two major clades (clade Ⅰ and clade Ⅱ):clade Ⅰ was composed of some specimens of Oujiang River,all the specimens of Hanjiang River and Jiulongjiang River,whereas all remaining populations fell in clade Ⅱ.The genetic distance between clade Ⅰ and clade Ⅱ was 0.036,while that between H.labeo and H.medius was 0.027.The haplotype network analyses indicated that the populations of Hanjiang River and Jiulongjiang River had relatively high genetic variation with the rest rivers.The po-pulations of Hainan Island migrated northward to Moyangjaing River.Haplotypes of the rivers of Hainan Island and Moyangjang River had relatively higher genetic variation with the Yangtze River than Pearl River.The populations of Xiangjiang River had no genetic variation with the populations of Guijiang River and Liujiang River.Analysis of molecular variance (AMOVA) indicated that the genetic variance mainly presented in individuals between geographical regions.The genetic variation of populations among regions was 71.2％,the genetic variation among populations within regions was 16.6％,and that within populations within the regions was 12.2％,indicating that most of the genetic variations resided in the populations among regions.The results of mismatch distribution and tests of neutrality suggested that in all populations,H.labeo,H.medius,clade Ⅰ and clade Ⅱ were relatively stable.关键词
唇(鱼骨)/间(鱼骨)/遗传结构/遗传多样性/COI基因/ND5基因/中国南部Key words
Hemibarbus labeo/Hemibarbus medius/genetic structure/genetic diversity/COI gene/ND5 gene/South China引用本文复制引用
蓝昭军,林龙峰,赵俊..基于COI和ND5序列的中国南部唇(鱼骨)和间(鱼骨)群体遗传结构[J].应用生态学报,2017,28(4):1377-1386,10.基金项目
本文由国家自然科学基金项目(31372178)、公益性行业(农业)科研专项(201303048)、广东省科技计划项目(2015A070706009)和国家科技基础条件平台工作重点项目(2005DKA21402)资助 This work was supported by the National Natural Science Foundation of China (31372178),the Special Fund for Agro-scientific Research in the Public Interest (201303048),the Project of Science and Technology Research of Guangdong Province (2015A070706009) and the National Key Project of Science-technology Basic Condition Platform (2005DKA21402). （31372178）
