湖北医药学院学报2016，Vol.35Issue(5)：435-438,4.DOI:10.13819/j.issn.1006-9674.2016.05.002
HLA-A*0201限制性GPC3表位肽的初步预测与筛选
Prediction and Preliminary Screening of HLA-A*0201 Restricted Epitope Peptides of Human GPC3
摘要
Abstract
Objective To screen the advantage epitope peptides of HLA-A * 0201 restricted human GPC3,so as to provide the new target for hepatocellular carcinoma (HCC) immunotherapy.Methods Three approved epitope prediction websites were used to predict the epitopes primarily,T2 cells binding assay were performed to screen the optimal epitopes.Results Total 13 epitopes were selected through the three prediction websites,then peptides were synthesized by standard solid phase chemistry.T2 cells binding assay demonstrated that 4 optimal peptides showed high affinity with HLA-A2.Conclusion Combined epitope prediction websites with T2 cells bindin assay could accomplish a preliminary screening of HLAA * 0201 restricted epitope peptides of human GPC3,which could be used as optional targets for HCC specific CTLs therapy.关键词
肝癌/GPC3/表位肽/肿瘤特异性抗原Key words
Hepatocellular carcinoma/Glypican-3/Epitope peptide/Tumor specific antigen引用本文复制引用
胡培,刘莉,万松,孟忠吉..HLA-A*0201限制性GPC3表位肽的初步预测与筛选[J].湖北医药学院学报,2016,35(5):435-438,4.基金项目
湖北医药学院优秀中青年科技创新团队项目(2014CXG05) （2014CXG05）
湖北省自然科学基金项目(2014CFB645) （2014CFB645）
太和医院科研专项(2014KF19) （2014KF19）
