湖北医药学院学报2016，Vol.35Issue(5)：449-454,457,7.DOI:10.13819/j.issn.1006-9674.2016.05.005
固醇调节元件结合蛋白(SREBP)的分子进化分析
Molecular Evolution of SREBP in Metazoa
摘要
Abstract
Objective To explore the origin and evolution of SREBP by phylogenetic analysis.These analysis can give useful information for its functions involving in fat metabolism related disease.Methods BLASP searches were performed to identify SREBP related proteins in public database NCBI and Ensembl.Primary structures of these proteins were analyzed by software in EXPASY and HPRD databases.MUSCLE program was used for the alignment and conservation.Phylogenetic tree was reconstructed by MEGA6.Results There were two copies of SREBP gene in vertebrates but only,one gene in invertebrates and basal metazoa.The bHLH DNA binding motif was highly conserved in all examined species.The proteolytic cleavage site S2P was only conserved in vertebrates.Conclusion The conserved function of SREBP in fat metabolism was originated from basal metazoa.The SREBP duplication event occurred before the speciation bony fish.关键词
SREBP/固醇代谢/脂类代谢/分子进化Key words
Molecular evolution/SREBP/Cholesterol/Fatty acid引用本文复制引用
李琛,郑兰兰,张璟璇,杨念,曹风军,汪选斌,张勇洪..固醇调节元件结合蛋白(SREBP)的分子进化分析[J].湖北医药学院学报,2016,35(5):449-454,457,7.基金项目
湖北省高等学校优秀中青年科技创新团队计划(T201510) （T201510）
湖北省教育厅科学技术研究项目(B2014046,B2015471) （B2014046,B2015471）
湖北医药学院优秀中青年团队项目(2014CXG03) （2014CXG03）
湖北省重点学科建设项目(2014XKJSXJ18) （2014XKJSXJ18）
湖北医药学院自然科学研究计划(2014QDJZR06) （2014QDJZR06）
湖北省卫生计生西医类一般项目(WJ2015MB291) （WJ2015MB291）
湖北省自然科学基金青年基金(2015CFB212) （2015CFB212）
