针刺研究2017，Vol.42Issue(2)：107-113,7.DOI:10.13702/j.1000-0607.2017.02.003
电针“胃脘下俞”对2型糖尿病大鼠胰岛形态及胰腺胰高血糖素样肽1受体的影响
Effect of Electroacupuncture at "Weiwanxiashu" (EX-B 3) on Islet Morphology and the Expression of Pancreatic Glucagon-like Peptide-1 Receptor in Type 2 Diabetes Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) on pancreatic glucagon-like peptide-1 receptor (GLP-1 R),pancreatic and duodenal homeobox 1 (PDX-1) protein expression and blood glucose in type 2 diabetes rats,so as to explore the underlying mechanism of EA treatment in improving type 2 diabetes.Methods Sprague-Dawley rats were randomly divided into blank control group,model group,"Weiwanxiashu" (EX-B 3) group,"Xinshu" (BL 15) group,and "Shenshu" (BL 23) group,12 rats in each group.Diabetes model was established by feeding the rat with high fat and high sugar diet combined with intraperitoneal injection of streptozotocin (35 mg/kg).All the EA groups received 2 Hz,2 mA continuous wave treatment for 20 min everyday,6 times per week lasting for 4 weeks.Fasting blood glucose was measured by Roche glucometer before and after treatment.Hematoxylin and eosin (HE) staining and Masson staining were used to detect pancreas morphology.GLP-1 R and PDX-1 protein expressions in the pancreas were detected by Western blot.Results Compared to the blank control group,fasting blood glucose was significantly increased in the model group(P＜0.01),accompanied with shrunken islet area,reduced nucleus counts of islet β cells,and compensatorily enlarged β cell nucleus.Compared to the model group,EA intervention significantly reduced fasting blood glucose level only in the EX-B 3 group (P＜0.05),partly restored pancreas morphology and nucleus counts of islet β cells in the EX-B 3,BL 15,and BL 23 groups.Compared to the blank control group,GLP-1 R and PDX-1 expressions were decreased in the model group (P＜0.01),while EA treatment could obviously increase GLP-1 R expression in the EX-B 3(P＜0.01),BL 15 (P＜0.01) and BL 23 (P＜0.05) groups compared with the model group.The expression of GLP-1 R in the BL 15 group was the highest among the three EA groups (P＜0.05,P＜0.01),and that in the EX-B 3 group was higher than in the BL 23 group (P＜0.05).There were no signifincant differences in the expression of PDX-1 protein among the three EA groups (P＞0.05).Conclusion EA treatment at EX-B 3 can reduce blood glucose via regulating pancreas function,increasing pancreatic GLP-1 R expression,and partly restoring the morphology of pancreas.关键词
电针/2型糖尿病/胰腺/胰高血糖素样肽1/胰腺十二指肠同源盒基因Key words
Electroacupuncture/Type 2 diabetes/Pancreas/Glucagon-like peptide-1/Pancreatic and duodenal homeobox分类
医药卫生引用本文复制引用
曹昺焱,李瑞,田环环,贾宁,胡晓刚,马艳佳,王跃颖..电针“胃脘下俞”对2型糖尿病大鼠胰岛形态及胰腺胰高血糖素样肽1受体的影响[J].针刺研究,2017,42(2):107-113,7.基金项目
教育部科学技术研究重大项目(No.313010) （No.313010）
