针刺研究2017，Vol.42Issue(2)：114-118,5.DOI:10.13702/j.1000-0607.2017.02.004
电针治疗大鼠弱精子症的实验研究
Experimental Study for the Treatment of Asthenozoospermia by Electroacupuncture in Rats
摘要
Abstract
Objective To investigate the effects of different electroacupuncture (EA) parameters for the treatment of asthenozoospermia in rats.Methods One hundred and five male Sprague-Dawley rats were randomly divided into 2 Hz-EA treatment daily in 3 d group (n=9),sham-EA group (n =10),model group (n =10);2 Hz-EA treatment every other day in 5 d group,sham-EA group,model group (8 rats in each group);2 Hz-EA treatment every other day in 9 d group,sham-EA group,model group (10 rats in each group);100 Hz-EA treatment every other day in 9 d group (n =7),sham-EA group (n =8),model group(n =7).Asthenozoospermia model was established by intragastric administration of ornidazole (ORN,400 mg · kg-1 · d-1) once daily till the end of treatment.EA treatments (2 Hz or 100 Hz) were applied to "Shenshu" (BL 23,bilateral),"Zusanli" (ST 36,bilateral) for 30 min,intensity of 1-2-3 mA (increasing 1 mA per 10 min),once a day or once every other day for 3 times or 5 times.Sham-EA groups were treated with similar procedure except that the output leads of the stimulator were disconnected.The sperm density,viability,motility,the number of grade A sperm,and grade A+ B sperm were examined by computer-assisted sperm analysis.Results (1) 2 Hz-EA treatment daily in 3 d:compared with the model group and the sham-EA group,2 Hz-EA treatment once daily had no significant effect on all of the sperm motility indexes in the asthenozoospermic rats (P＞0.05).(2) 2 Hz-EA treatment every other day in 5 d:compared with the model group,EA treatment could increase the sperm motility (P＜0.05),the number of grade A sperm (P＜0.05),and the number of grade A+ B sperm (P＜0.05) in the asthenozoospermic rats.However,compared with the sham-EA group,EA treatment could only improve the number of grade A + B sperm (P＜0.05).(3) 2 Hz-EA treatment every other day in 9 d:compared with both the model group and the sham-EA group,EA treatment could markedly improve the sperm viability (P＜0.001),the sperm motility (P＜0.001),the number of grade A sperm (P＜0.001),and the number of grade A+ B sperm (P＜0.001) in the asthenozoospermic rats.(4) 100 Hz-EA treatment every other day in 9 days:compared with both the model group and the sham-EA group,all of the sperm indexes in the asthenozoospermic rats including the sperm viability (P＜0.001 vs.the model group,P＜0.05 vs.the sham-EA group),the sperm motility (P＜0.001 vs.the model group,P＜0.01 vs.the sham-EA group),the number of grade A sperm (P＜0.01) and the number of grade A+B sperm (P＜0.01) also could be improved after EA treatment.Unexpectedly,none of the EA treatment had significant influence on the sperm density in the asthenozoospermic rats.Conciusion Both 2 Hz-EA and 100 Hz-EA treatment once every other day for 5 times in 9 d had a therapeutic effect on asthenozoospermia by improving the sperm viability and the sperm motility in the rats.关键词
弱精子症/电针/计算机辅助精子分析/精子运动Key words
Asthenozoospermia/Electroacupuncture/Computer-assisted sperm analysis/Sperm motility分类
医药卫生引用本文复制引用
金滋润,柳博珩,蔡捷,景向红,朱兵,邢国刚..电针治疗大鼠弱精子症的实验研究[J].针刺研究,2017,42(2):114-118,5.基金项目
国家卫生和计划生育委员会行业专项基金(No.201302013-01) （No.201302013-01）
中国中医科学院针灸研究所开放选题项目(No.ZZ 04004) （No.ZZ 04004）
国家自然科学基金项目(No.81371237、81072951、61527815) （No.81371237、81072951、61527815）
国家重点基础研究发展计划(“973”计划)课题(No.2013 CB 531905) （“973”计划）
