针刺研究2017，Vol.42Issue(2)：125-130,6.DOI:10.13702/j.1000-0607.2017.02.006
电针对脑缺血大鼠丘脑腹后外侧核神经生长因子和生长休止特定蛋白7表达的影响
Effect of Electroacupuncture Intervention on Expressions of Nerve Growth Factor and Growth Arrest-specific Protein 7 in Ventral Posterolateral Thalamic Nucleus in Cerebral Ischemia Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA) on expressions of nerve growth factor(NGF) and growth arrest-specific protein 7(Gas 7) in the right ventral posterolateral nucleus(VPL) in the focal cerebral ischemia (FCI) rats,so as to explore its possible mechanism underlying improvement of secondary injury of FCi.Methods Forty-eight SpragueDawley rats were randomly divided into normal group,sham operation group,model group and electroacupuncture (EA) group (n=12 in each group) by random number table.The FCI model was made by occlusion of the right middle cerebral artery (MCAO) with thread embolus.One week after MCAO,EA(2 Hz,2 V) was performed on "Baihui"(GV 20) and left "Zusanli" (ST 36) for 30 min,once daily for successively 21 days.The expressions of NGF and Gas 7 in the right VPL were detected by immunohistochemistry (n =6 in each group) and Western blot (n =6 in each group),respectively;meanwhile Nissl staining was conducted to show VPL neurons.Results Nissl staining showed that the structure of right VPL was clear and complete,and the nuclei were centered and clear in the normal group and sham operation group;the VPL neurons were deeply stained,and the nuclei were pyknotic in the model group;the morphology of neurons in the EA group was similar to that of the normal group.The results of immunohistochemistry and Western blot were consistent.Expressions of NGF and Gas 7 proteins in the right VPL were not of significant differences between the sham operation group and the normal group (P＞0.05).Compared with the normal group,the expressions of NGF and Gas 7 proteins in the VPL of the ischemic side were significantly increased in the model group(P＜0.05).After treatment,the expression levels of NGF and Gas 7 proteins were further up-regulated in the EA group in comparison with the model group (P＜0.05).Conclusion The enhanced expressions of NGF and Gas 7 in the ischemic VPL of FCI rats may be involved in the neuroprotection and repairing;EA can significantly up-regulate the expressions of NGF and Gas 7 in VPL of the ischemic side,which may contribute to its effect in improving secondary thalamic impairment of FCI.关键词
电针/脑缺血/腹后外侧核/神经生长因子/生长休止特定蛋白7Key words
Electroacupuncture/Cerebral ischemia/Ventral posterolateral nucleus/Nerve growth factor/Growth arrest-specific protein 7分类
医药卫生引用本文复制引用
丁见,吴锋,缪化春,黄锐,熊克仁..电针对脑缺血大鼠丘脑腹后外侧核神经生长因子和生长休止特定蛋白7表达的影响[J].针刺研究,2017,42(2):125-130,6.基金项目
皖南医学院中青年科研基金项目(No.WK 201509) （No.WK 201509）
皖南医学院校重点科研项目培育基金项目(No.WK 2016 Z 09) （No.WK 2016 Z 09）
安徽省青年自然科学基金(No.1608085 QH 223) （No.1608085 QH 223）
安徽省名师工作室(No.2014 msgzs 152) （No.2014 msgzs 152）
