针刺研究2017，Vol.42Issue(2)：131-135,5.DOI:10.13702/j.1000-0607.2017.02.007
不同腧穴配伍电针防治大鼠心肌缺血损伤作用的比较
Comparison of Protective Effect of Electroacupuncture on Myocardial Ischemia Injury Between Different Acupoint Formulas in Rats
摘要
Abstract
Objective To observe the different effects of electroacupucnture of "Neiguan" (PC 6) grouping with other acupoints on myocardial ischemia (MI) injury,so as to provide evidence for clinical acupoint formulas.Methods Forty Wistar male rats were randomly divided into normal,model,EA 1 [bilateral "Neiguan" (PC 6),bilateral "Zusanli" (ST 36),"Guanyuan" (CV 4)],and EA 2 [bilateral "Neiguan" (PC 6),bilateral "Shenmen" (HT 7),"Danzhong" (CV 17)] groups,10 rats in each group.The MI model was established by subcutaneous injection with ISO solution(5 mL/kg),once per day for 7 days.Both EA 1 and EA 2 groups were subjected to EA (2 Hz/100 Hz,1 mA) for 10 min before subcutaneous injection,once daily for 21 days.Electrocardiogram (ECG) was recorded after treatment.Serum cTnT was detected by biochemistry.SOD,GSH-Px and MDA in myocardial tissues of left ventricular were measured by ELISA,and the ultrastructural changes of myocardial cells in the left ventricular were observed by electron microscope.Results Compared with the normal group,the amplitude of S-T segment and HR of ECG,cTnT and MDA contents were significantly increased in the model group (P＜0.01) accompanied with seriously destroyed ultrastructure in the myocardial tissue,while SOD and GSH-Px levels were lower than those in the normal group (P ＜ 0.01).However,EA treatments reversed all the abnormal changes in the model group (,P＜0.05).There was no significant difference between EA 1 and EA 2 groups in the amplitude of S-T segment and HR (P＞0.05);cTnT and MDA levels in the EA 1 group were lower than those in the EA 2 group (F＜0.05),while SOD and GSH-Px levels were higher than those in the EA 2 group (P＜0.05),accompanied with lighter degree of ultrastructure damage in the myocardial tissue in the EA 1 group.Conclusion EA can prevent myocardial ischemic injury,and the effect of EA 1 treatment was better than that of EA 2.关键词
心肌缺血/电针/内关穴/腧穴配伍Key words
Myocardial ischemia/Electroacupuncture/"Neiguan"(PC 6)/Acupoint formulas分类
医药卫生引用本文复制引用
谢俊,陈泽斌,吴松,梁凤霞,郝青,陈茜,王华..不同腧穴配伍电针防治大鼠心肌缺血损伤作用的比较[J].针刺研究,2017,42(2):131-135,5.基金项目
国家自然科学基金(No.81273865、81473789、81403460) （No.81273865、81473789、81403460）
