针刺研究2017，Vol.42Issue(2)：153-158,6.DOI:10.13702/j.1000-0607.2017.02.012
穴位贴敷对慢性哮喘小鼠气道重塑及转化生长因子β1/Smad3表达的影响
Inhibitory Effect of Acupoint Application on Airway Remodeling and Expression of TGF-β 1/ Smad 3 in the Lung Tissue of Chronic Asthma Mice
摘要
Abstract
Objective To observe the effect of acupoint application on transforming growth factor (TGF)-β 1/Smad 3 signaling pathway,so as to reveal its mechanism underlying improvement of airway remodeling in chronic asthma mice.Methods BALB/c mice were randomly divided into normal group,model group,sham acupoint application group (sham group),acupoint application group,and dexamethasone group (n =8 in each group).The chronic asthma model was established by intraperitoneal injection of ovalbumin sensitization.The acupoint application group was treated with plaster composed with White Mustard Seed,Rhizoma Corydalis,Unprocessed Radix Kansui,and Herba Asari,on bilateral "Feishu" (BL 13),"Xinshu" (BL 15) and "Geshu" (BL 17) points,6 hours a day for 14 days.The sham group was treated with plaster containing vaseline on bilateral BL 13,BL 15 and BL 17,6 hours a day for 14 days.The dexamethasone group was treated with dexamethasone 1 mg · kg-1 · day-1 intraperitoneally,once daily for 14 days.At the end of treatment,airway resistance was detected by mouse lung function instrument.Patho-logical changes of airway were observed under light microscope after hematoxylin-eosin staining,and the morphological parameters were measured by image analysis software,lmmunohistochemistry method was used to detect the expression of TGF-β 1 and Smad 3 in the lung tissue of mice.Results Compared with the normal group,the airway resistance,the total bronchial wall area (WAt)/lumen perimeter (Pi),the smooth muscle wall area (WAm)/Pi,and the expression of TGF-β 1 and Smad 3 in the lung tissues were significantly higher in the model and sham groups (P＜0.01).Compared with the model group and the sham group,the airway resistance,WAt/Pi,WAm/Pi,the expression of TGF-β 1 and Smad 3 in the lung tissues were decreased in the acupoint application group and the dexamethasone group (P＜0.05).There were no significant differences between the acupoint application group and the dexamethasone group in the above mentioned indexes (P＞0.05).Conclusion Acupoint application can improve airway remodeling by down-regulating the expression of TGF-β 1/Smad 3 in the airway of chronic asthmatic mice.关键词
穴位贴敷/慢性哮喘/气道重塑/转化生长因子β 1/Smad 3通路Key words
Acupoint application/Chronic asthma/Airway remodeling/TGF-β 1/Smad 3 pathway分类
医药卫生引用本文复制引用
刘成勇,秦珊,刘兰英,贺亚敏,王和生,吴文忠,陈栋,张聪..穴位贴敷对慢性哮喘小鼠气道重塑及转化生长因子β1/Smad3表达的影响[J].针刺研究,2017,42(2):153-158,6.基金项目
国家自然科学基金(No.81674065) （No.81674065）
国家自然科学青年基金(No.81102632) （No.81102632）
江苏省人力资源与社会保障厅项目(No.2014-WSN-015) （No.2014-WSN-015）
“十二五”国家科技支撑项目(No.2015 BAI 04 B 11) （No.2015 BAI 04 B 11）
