中国动脉硬化杂志2017，Vol.25Issue(4)：332-336,5.
D-半乳糖诱导的心肌细胞自噬下降与microRNA的表达变化有关
The decreasing of autophagy in cardiomyocytes induced by D-galactose is related to the changes of microRNA expression
摘要
Abstract
Aim To investigate the relationship between microRNA (miRNA) and autophagy in aged myocardial cells induced by D-galactose.Methods The D-galactose with 0,4,8,16,20 g/L was used to induce the rat myocardial cells to senescence respectively,and being stimulated 3,6,9 days later,the aging level of H9c2 was detected by the beta-galactosidase staining;the formation of autophagosomes in aging rat cardiomyocytes induced by D-galactose was detected by electron microscope;the mRNA expression level of miRNA-30a,miRNA-126,miRNA-204 and protein expression level of LC3B Ⅱ/LC3B Ⅰ in aging rat cardiomyocytes and H9C2 cells was detected by quantitative real-time(qRT-PCR) and Western blot methods respectively.Results Compared with the control group,the cell staining was gradually deepened with the increase of drug concentration and the stimulation days,and when D-galactose concentration was 8 g/L for 9 days,cell staining was the most obvious;electron microscopy showed that the autophagy in aging cardiomyocytes induced by D-galactose was decreased;Western blot results showed that the level of LC3B Ⅱ/LC3B Ⅰ in aging cardiomyocytes was reduced;qRT-PCR results showed that in the aging cardiomyocytes group the expression levels of miRNA-30a and miRNA-126 were decreased and miRNA-204 was significantly increased.Conclusion In the process of the autophagy level decreasing in senescent cardiomyocytes induced by D-galactose,there exists a reducing in miRNA-30a,miRNA-126 expression level and an increasing in miRNA-204 expression level,the differential expression of miRNAs may be an important mechanism of autophagy in senescent cardiomyocytes.关键词
衰老心肌细胞/自噬/microRNAKey words
Aging cardiomyocytes/Autophagy/microRNA分类
医药卫生引用本文复制引用
朱光荣,姜怡邓,杨晓玲,丁宁,杨安宁,杨松昊,王楠,侯苗苗,谢琳,蒋袁絮,田珏..D-半乳糖诱导的心肌细胞自噬下降与microRNA的表达变化有关[J].中国动脉硬化杂志,2017,25(4):332-336,5.基金项目
国家自然科学基金资助项目(81560242、81360063) （81560242、81360063）
宁夏教育厅项目(NGY2015092) （NGY2015092）
