中国动脉硬化杂志2017，Vol.25Issue(4)：337-342,6.
高迁移率族蛋白1及其受体对大鼠骨髓间充质干细胞增殖及细胞因子水平的影响
Effects of high mobility group box 1 and its receptor on proliferation and cytokine levels of rat marrow mesenchymal stem cells
摘要
Abstract
Aim To investigate the effect of high mobility group box 1 (HMGB1) and its receptor on survival and cytokine levels of rat marrow mesenchymal stem cells.Methods Marrow mesenchymal stem cells were treated with HMGB1 at 25.0,50.0 and 100.0 μg/L for 24,48 and 72 hours.Cell proliferations were detected by MTT after different treatment.VEGF and bFGF were detected by ELISA.Expressions of receptor of advanced glycation end products (RAGE) and Toll-like receptor 4 (TLR4) in mesenchymal stem cells were detected by Western blot.Real-time PCR and Western blot were used to examine RAGE and TLR4 expression,which were to verify the efficiency of transfection with siRNA in marrow mesenchymal stem cells.MTT assay and ELISA were used to analyze proliferation and secretions of VEGF and bFGF in si-RAGE or si-TLR4 marrow mesenchymal stem cells.Results 25.0 μg/L HMGB1 could increase marrow mesenchymal stem cells proliferation (P＜0.05).25.0 and 50.0 μg/L HMGB1 could promote VEGF and bFGF secretion compared with control group (P＜0.05).The protein levels of RAGE and TLR4 in 25.0 μg/L HMGB1 group were significantly higher than those in other groups (P＜0.05).Marrow mesenchymal stem cells transfected with si-TLR4 antagonized the cell proliferation and secretion of VEGF,bFGF induced by HMGB1.Conclusion HMGB1 promote cell proliferation and secret VEGF,bFGF by binding to its TLR4 receptor.关键词
高迁移率族蛋白1/骨髓间充质干细胞/细胞因子Key words
High mobility group box 1/Marrow mesenchymal stem cells/Cytokine分类
医药卫生引用本文复制引用
刘志江,石蓓,束波,马帅..高迁移率族蛋白1及其受体对大鼠骨髓间充质干细胞增殖及细胞因子水平的影响[J].中国动脉硬化杂志,2017,25(4):337-342,6.基金项目
贵州省科技计划项目[黔科合SY字(2012)3109号] （2012）
遵义市科技计划项目[遵市科会社字(2014)82号] （2014）
