中国动脉硬化杂志2017，Vol.25Issue(4)：343-346,4.
巴西苏木素调控自噬对高糖诱导血管内皮细胞损伤的影响
Effects of autophagy on high glucose induced injury of vascular endothelial cells in Brazilin
摘要
Abstract
Aim To study the protective effect and possible mechanism of Brazilin on high glucose induced vascular endothelial cell injury,and to provide experimental basis for the treatment of diabetic macrovascular disease with traditional Chinese medicine hematoxylin.Methods Human umbilical vein endothelial cells (HUVEC) were cultured,the concentration of Brazilin was determined by MTT method,high glucose induced injury after joining Brazilin,autophagy body was observed by transmission electron microscopy (TEM),tubule formation test was used to observe angiogenesis,the protein expression of autophagy related genes BECLIN1,LC3-Ⅱ and p62 was detected by Western blot.Results The concentration of Brazilin was 15 mg/L.Transmission electron microscopy showed that the number of autophagic cells in high glucose treatment group was less than that in normal group,but the number of autophagy in the cells of Brazilin group was higher than that in normal group and high glucose treatment group.The expression of BECLIN 1 and LC3-Ⅱ protein in high glucose treated group was lower than that in normal group,the expression of BECLIN 1 and LC3-Ⅱ protein in Brazilin group was higher than that in high glucose treatment group (P＜0.05);the expression of p62 protein in high glucose treated group was higher than that in normal group,the expression of p62 protein in Brazilin group was lower than that in normal group and high glucose treatment group (P＜0.05).Conclusion Brazilin may play a role of protection in high glucose induced vascular endothelial cell injury through regulating autophagy.关键词
巴西苏木素/糖尿病大血管病变/自噬Key words
Brazilin/Diabetic macrovascular disease/Autophagy分类
医药卫生引用本文复制引用
刘婷,佟晓哲..巴西苏木素调控自噬对高糖诱导血管内皮细胞损伤的影响[J].中国动脉硬化杂志,2017,25(4):343-346,4.基金项目
辽宁省自然科学基金资助项目(2015020343) （2015020343）
