中国动脉硬化杂志2017，Vol.25Issue(4)：365-368,4.
黄芩苷对ox-LDL诱导的人脐静脉内皮细胞损伤的保护作用
Protective effect of Baicalin on ox-LDL induced injury of human umbilical vein endothelial cells
康超 1张秋香 1赵美丽1
作者信息
- 1. 金乡县人民医院心内一科,山东省金乡县272200
- 折叠
摘要
Abstract
Aim To observe the protective effect of Baicalin on vascular endothelial cells induced by oxidized low density lipoprotein (ox-LDL).Methods In vitro cultured human umbilical vein endothelial cells (EAhy926) were divided into control group,ox-LDL group,Baicalin group and different concentrations of Baicalin (25,50 and 100 mg/L) +ox-LDL groups,cultured 24 h.Cell viability was detected by CCK-8,ELISA was used to detect the level of IL-6 and TNF-α in cell culture supernatant,Western blot was used to detect the expression of apoptosis related protein Bax and Bcl-2.Results Compared to the ox-LDL group,the cell survival rates of 50,100 mg/L Baicalin+ox-LDL groups were significantly increased (P＜0.05),the protein levels of both TNF-α and IL-6 in cell culture supematant were decreased significantly (P＜0.05),and the ratio of Bax/Bcl-2 decreased (P＜ 0.05).Conclusion Baicalin protects against endothelial cell injury,which may be achieved by inhibiting inflammatory factors,reducing apoptosis and enhancing cell activity.关键词
动脉粥样硬化/氧化型低密度脂蛋白/黄芩苷/人脐静脉内皮细胞Key words
Atherosclerosis/Oxidized low density lipoprotein/Baicalin/Human umbilical vein endothelial cells分类
医药卫生引用本文复制引用
康超,张秋香,赵美丽..黄芩苷对ox-LDL诱导的人脐静脉内皮细胞损伤的保护作用[J].中国动脉硬化杂志,2017,25(4):365-368,4.
