中国动脉硬化杂志2017，Vol.25Issue(4)：373-377,5.
蛋白激酶C活性与糖尿病足截肢患者胫前动脉钙化积分的相关性
Correlation between protein kinase C activity and anterior tibial artery calcification score in patients with diabetic foot amputation
摘要
Abstract
Aim To observe the relationship between protein kinase C (PKC) activity and anterior tibial artery calcification score in patients with diabetic foot.Methods 60 diabetic patients hospitalized for above-knee amputation in the Department of Orthopedics,Affiliated Hospital of Jiangsu University were recruited from October 2012 to January 2016.The patients were categorized based on the severity of anterior tibial artery calcification,which was assessed by computed tomography (CT),into mild calcification group (0＜calcification score＜ 100,n =20),moderate stenosis group (100≤calcification score＜200,n=20) and severe calcification group (calcification score≥200,n=20).Baseline clinical data regarding sex,age,duration of diabetes mellitus,hypertension status,fasting plasma glucose (FPG),glycated hemoglobin (HbA1c),and lipid profile were collected before the amputation.Von kossa staining,PepTag(R) Assay and Western blot were then performed.Results Similarly with Von Kossa staining,CT scan results showed that calcium deposition in anterior tibial artery plaques of diabetic patients hospitalized for above-knee amputation were enlarged and calcium particles density in moderate and severe groups were stronger than that in mild group.The calcification in all calcification groups were showed as spotty calcification.PepTag(R) Assay,Western blot and Pearson correlation showed that anterior tibial artery calcification score in diabetic patients hospitalized for above-knee amputation was positively associated with PKC activity (r=0.931,P＜0.001).Further analysis indicated that alpha subtypes of PKC maybe played an important role to promote the evolution of calcification.Conclusion The activation of PKC subtypes may be related with the evolution of calcification in atherosclerotic plaques,so as to further target the mechanism of vascular calcification.关键词
糖尿病足/动脉粥样硬化/血管钙化/蛋白激酶CKey words
Diabetic foot/Atherosclerosis/Vascular calcification/Protein kinase C分类
医药卫生引用本文复制引用
李丽华,叶斐,付先利,吴建农,严金川,邵晨,王中群..蛋白激酶C活性与糖尿病足截肢患者胫前动脉钙化积分的相关性[J].中国动脉硬化杂志,2017,25(4):373-377,5.基金项目
国家自然科学基金项目(81370408、81370409) （81370408、81370409）
江苏省自然科学基金项目(BK20131246) （BK20131246）
江苏省卫生厅项目(Q201308) （Q201308）
镇江市社会发展项目(SH2015038、SH2015023、SHW2015001) （SH2015038、SH2015023、SHW2015001）
卫生部核医学重点实验室、江苏省分子核医学重点实验室开放课题(KF201504) （KF201504）
江苏大学临床专项项目(JDLCZX001) （JDLCZX001）
