中国感染控制杂志2017，Vol.16Issue(3)：195-198,4.DOI:10.3969/j.issn.1671-9638.2017.03.001
产气肠杆菌携带基因blaNDM-1的质粒特征及基因环境分析
Characteristics and blaNDM-1 genetic environment of plasmid from Enterobacter aerogenes
摘要
Abstract
Objective To study plasmid-mediated transfer,plasmid replicon typing,and genetic environment of blaNDM-1 gene in Enterobacteraerogenes(E.aerogenes).Methods E.aerogenes HN-NDM0711 was used as the subject of this research,the transferable properties of plasmid were analyzed by conjugation testing,conjugant was performed stability testing,plasmid type was determined by PCR-based replicon typing (PBRT),downstream and upstream of blaNDM-1 were sequenced using chromosome walking method,genetic context was analyzed by BLASTN and BALSTP,as well as annotated using Vector NTI 11.5.1 software,sequence pipeline graph was made,the sequence was submitted to Genbank through software Banklt.Results The conjugation testing of E.aerogenes pHN-NDM0711 was positive,after positive conjugant was conducted 4-day passage,minimal inhibitory concentrations (MICs) of imipenem and meropenem to all the cloned strains didn't change,blaNDM-1 were all positive.The replicon type was IncA/C;blaNDM-1 gene was localized between ISAba14 and IS91,at upstream of the blaNDM-1,class 1 integron and Tn3 transposon were identified,class 1 integron contained a new mosaic structure of a drug-resistant resistance gene cassette.Conclusion E.aerogenes pHN-NDM071 1,bearing blaNDM-1 gene in IncA/C plasmid,derived from gene recombination under different antimicrobial selection pressure.Antimicrobial use in clinical,industrial and agricultural area should be strictly controlled,so as to reduce the emergence of such bacteria.关键词
产气肠杆菌/NDM-1/接合实验/质粒复制子分型/β-内酰胺酶Key words
Enterobacter aerogenes/NDM-1/conjugation testing/plasmid replicon typing/β-lactamase分类
医药卫生引用本文复制引用
肖伟强,许青霞,李铁鹏,王智中,潘军,姚新伟,常彦敏,孙明月..产气肠杆菌携带基因blaNDM-1的质粒特征及基因环境分析[J].中国感染控制杂志,2017,16(3):195-198,4.基金项目
河南省卫生厅科技攻关项目(201503192) （201503192）
