中国林副特产Issue(2)：4-8,5.DOI:10.13268/j.cnki.fbsic.2017.02.002
玉米杂交种子纯度的SSR分子标记与盐溶蛋白检测法的比较研究
The Comparison Research of SSR Molecular Marker And Salt-Soluble Protein PAGE In Identification of Maize Seed Purity
摘要
Abstract
In this study,Took 9 maize hybrids with larger area as material,salt-soluble protein PAGE and SSR analysis were used in Comparing test of hybrids purity.The results showed that 8 hybrid could be distinguished by salt-soluble protein PAGE,and it was simple,quick,accurate,stable and cheap characteristics and can be applied in a large scale.One combinations won,t identified by salt-soluble protein PAGE because of the limitation of the low variation among salt-soluble proteins.40 primerswere used in SSR analysis and only 3 primer combinations could identify all the 9 hybrid corn combinations,But it canct be applied in a large scale on account of its high expense.In conclusion,salt-soluble protein PAGE can be used in most cases and SSR can be used in identifying difficultly.关键词
种子纯度/盐溶蛋白电泳/SSR分子标记Key words
Seed Purity/Salt-soluble protein PAGE/SSR molecular markers分类
农业科技引用本文复制引用
王晓梅,李贵全,索修改,傅迎军,王燕平,王晓东,孙殷会,张庆娜,白艳凤,徐德海,温义昌..玉米杂交种子纯度的SSR分子标记与盐溶蛋白检测法的比较研究[J].中国林副特产,2017,(2):4-8,5.基金项目
国家科技支撑计划项目(2014BAD01B01) （2014BAD01B01）
牡丹江市科学技术计划项目(Z2015n0011) （Z2015n0011）
