中国美容医学2017，Vol.26Issue(4)：61-66,6.
骨髓间充质干细胞双向诱导分化构建血管化组织工程骨的实验研究
Engineering Vascularized Bone Graft with Osteogenic and Angiogenic Lineage Differentiated Bone Marrow Mesenchymal Stem Cells
张蓉 1高瞻 1高伟1
作者信息
- 1. 新疆军区总医院颌面外科, 新疆 乌鲁木齐 830000
- 折叠
摘要
Abstract
Objective We report a novel strategy to engineer vascularized bone grafts with osteogenic and angiogenic lineage differentiated marrow mesenchymal stem cells(MSCs). Methods MSCs were expanded to form an osteogenic cell sheet using a continuous culture method and a scraping technique under osteogenic culture conditions. Another portion of MSCs was directed to differentiate into highly proliferative endothelial progenitor cells (EPCs), which were then seeded onto the cell sheets. Cell sheet–EPCs complexes were implanted subcutaneously in nude mice. Cell sheets without EPCs were also implanted as a control. The mice were sacrificed, and the samples were harvested for evaluation consisting of micro-CT scanning, histological analysis and scanning electronic microscopy 4 and 8 weeks after implantation. Results Cell sheets were composed of viable cells and extracellular matrix and showed apparent mineralization. The obtained EPCs could express the specific antigen marker of CD31 and form capillary-like structures in vitro. The osteogenic cell sheet–EPCs complexes yielded well-vascularized bone grafts 4 and 8 weeks after implantation. Both bone density and vascular density were significantly higher in the cell sheet–EPCs complex group than in the control group. Conclusion The results demonstrated that the introduction of EPCs could not only generate a vascular network but also increase bone formation for cell sheet- based bone engineering.关键词
骨组织工程/细胞膜片技术/血管化/间充质干细胞/血管内皮前体细胞Key words
bone tissue engineering/cell sheet/vascularization/mesenchymal stem cell/endothelial progenitor cells分类
生物科学引用本文复制引用
张蓉,高瞻,高伟..骨髓间充质干细胞双向诱导分化构建血管化组织工程骨的实验研究[J].中国美容医学,2017,26(4):61-66,6.
