中国麻业科学2017，Vol.39Issue(1)：12-18,7.
亚麻类萌发素蛋白基因LuGLP1-13的克隆及表达分析
Cloning and Expression Analysis of LuGLP1-13 Gene of Flax
摘要
Abstract
Germin-like proteins (GLPs) is a kind of defense response gene,which plays an important role in the process of plant disease resistance.In the previous study,a GLPs gene (Lus10003267) was significantly up-regulated under saline-alkaline stress by digital gene expression in flax,suggesting that this gene may be participated in saline-alkaline stress response.In this study,the Lus10003267 gene was cloned by RT-PCR and named as LuGLP1-13.The CDS of this gene was 687bp,encoding 228 amino acids.Bioinformatics analysis showed that the molecular weight of the protein was 24.3ku,the isoelectric point was 5.77,and the unstable coefficient was 23.64,which was a relatively stable acidic protein.LuGLP1-13 has a signal peptide sequence,which is a kind of extracellular proteins.Multiple sequence alignment and phylogenetic analysis showed that the protein has a typical GLPs family characteristic,and it had the closest relationship with Ricinus communis,Populus trichocarpa and Populus euphratica GLPs.The results of qRT-PCR showed that the LuGLP1-13 gene was significantly induced under saline-alkaline stress (especially the alkaline salt stress),suggesting that this gene may be involved in the response process to saline-alkaline stress.This study provided theoretical basis for further study on the function of LuGLP1-13 gene and the molecular me.chanism of saline-alkaline tolerance in flax.关键词
亚麻/类萌发素蛋白/基因克隆/盐碱胁迫/表达分析Key words
flax/germin-like proteins/gene cloning/saline-alkaline stress/expression analysis分类
农业科技引用本文复制引用
于莹,袁红梅,程莉莉,赵东升,陈晶,吴建忠,张树权,吴广文..亚麻类萌发素蛋白基因LuGLP1-13的克隆及表达分析[J].中国麻业科学,2017,39(1):12-18,7.基金项目
黑龙江省农业科学院博士科研启动金(201507-41) （201507-41）
国家麻类产业技术体系(CARS-19-S03) （CARS-19-S03）
