中国农业科技导报2017，Vol.19Issue(4)：119-127,9.DOI:10.13304/j.nykjdb.2016.449
龙陵铁皮石斛花色苷的提取工艺及抑菌活性研究
Extraction and Anti-microbial Activities of Anthocyanins from Longling Dendrobium candidum
摘要
Abstract
Taking Dendrobium candidum in Longling County of Yunnan Province as experimental material,this study obtained anthocyanins with solvent-extraction method.The effects of ethanol volume fraction,material-liquid ratio,extraction temperature,pH,extraction time on the extraction rate of anthocyanins were studied by single fator experiment;and the best extraction technology condition was obtained through technology optimization by orthogonality experimental method.Besides,bacteriostasic activities of anthocyanins from Dendrobium candidum inhibiting Escherichia coli,Staphyloccocus aureus,Bacillus subtilis were also studied.Results showed that the optimal condition were as following:ethanol 60％,material-liquid ratio 1 g ∶20 mL,extraction temperature 4℃,pH 3,extraction time 120 min,twice extraction time,and the total anthocyanins yield 3.12 mg/g FW.The minimum inhibitory mass concentration(MIC) on Escherichia coli,Staphyloccocus aureus,Bacillus subtilis were 9.75 mg/L,4.88 mg/L and 1.22 mg/L,respectively.And the minimum bactericidal concentration(MBC) were 9.75 mg/L,4.88 mg/L,2.44 mg/L,respectively.关键词
铁皮石斛/花色苷/提取/抑菌活性Key words
Dendrobium candidum/anthocyanin/extraction/anti-microbial activity分类
轻工纺织引用本文复制引用
李晓娇,晏爱芬,杨丽华,何健民,侯洪波..龙陵铁皮石斛花色苷的提取工艺及抑菌活性研究[J].中国农业科技导报,2017,19(4):119-127,9.基金项目
保山市科技计划项目(2014kj61) （2014kj61）
云南省教育厅科研基金项目(2012Y260) （2012Y260）
保山市中青年学术和技术带头人项目bszqnxshjsdtr2016(03)]资助. （03）
