中国兽医科学2017，Vol.47Issue(4)：403-410,8.DOI:10.16656/j.issn.1673-4696.2017.04.001
VP7-CTB融合蛋白在拟南芥种子中的表达及免疫原性分析
Expression and immunogenicity analysis of VP7-CTB fusion protein in transgenic Arabidopsis thaliana seeds
摘要
Abstract
To research and develop rotavirus vaccine production in plant,the neutralization antigen gene VP7 was fused with CTB gene,and pPHAP1301-his-VP7-liner-CTB expression vecter was constructed and transformed into Arabidpsis thaliana.The his-VP7-liner-CTB was analyzed by RT-PCR,Westernblot and ELISA.Further,the protective response of plant derived his-VP7-liner-CTB in mice was investigated by oral immunizations.The results showed that pPHAP1301-his-VP7-liner-CTB expression vecter was successfully constructed and transformed into Arabidopsis thaliana.Molecular analysis revealed that his-VP7-liner-CTB was efficiently expressed in plant seeds.The account of VP7 was 0.39％ in the sample of TSP that was expressed in Arabidopsis thaliana containing his-VP7-liner-CTB.A high level VP7 (56.25 μ g) protein was expressed in each gram of transgenic seeds.Mice was immunized and got immunogenicity with the TSP from trangenic seeds.The test of neonatal rats challenged with rotavirs his-VP7-liner-CTB proved that it had good protection for rotavirs and immune antibodies was effectively passed on to the offspring from mother rats.The results indicated that the VP7 protein produced by Arabidopsis thaliana was a candidate for a subunit vaccine specifically for the rotavirus strain.关键词
轮状病毒/VP7融合蛋白/拟南芥/免疫原性/疫苗Key words
rotavirs/VP7 fusion protein/Arabidopsis thaliana/immunogenicity/vaccine分类
农业科技引用本文复制引用
李余先,李校堃,官丽莉,周永刚,刘秀明,杨晶,郭咏昕,杨赫,王红雨,李海燕..VP7-CTB融合蛋白在拟南芥种子中的表达及免疫原性分析[J].中国兽医科学,2017,47(4):403-410,8.基金项目
国家高技术研究发展计划(863)项目(2011AA100606) （863）
