中国兽医科学2017，Vol.47Issue(4)：411-418,8.DOI:10.16656/j.issn.1673-4696.2017.04.002
新城疫病毒La Sota及F48E9毒株P和V及W蛋白C端功能域的原核表达及其多克隆抗体的制备
Prokaryotic expression of C-terminal domain of P, V and W protein of La Sota and F48E9 strains of Newcastle disease virus and preparation of their polyclonal antibodies
摘要
Abstract
The C-terminus of P,V and W genes of La Sota and F48E9 strains of Newcastle disease virus were amplified from La Sota and F48E9 strains.The six recombinant proteins were expressed prokaryotically and purified by Ni columns.And six polyclonal antibodies were generated.The results showed that the titers of six polyclonal antibodies were more than 1:2 560 tested by ELISA and the reactive specificity were high by Western-blotting using the polyclonal antibodies.The results indicated the polyclonal antibodies of P,V and W proteins of La Sota and F48E9 strains were successfullyprepared,and this laid the foundation for further studies on their biological functions.关键词
新城疫病毒/C端功能域/多克隆抗体Key words
Newcastle disease virus/C-terminal domain/polyclonal antibody分类
农业科技引用本文复制引用
王重阳,任善会,贾燕青,王相伟,高小龙,张淑霞,萧飒,王兴龙,杨增岐..新城疫病毒La Sota及F48E9毒株P和V及W蛋白C端功能域的原核表达及其多克隆抗体的制备[J].中国兽医科学,2017,47(4):411-418,8.基金项目
国家自然科学基金资助项目(31572538) （31572538）
国家蛋鸡产业技术体系项目(CARS-41-S23) （CARS-41-S23）
