中国兽医科学2017，Vol.47Issue(4)：481-488,8.DOI:10.16656/j.issn.1673-4696.2017.04.013
广西猪圆环病毒2型全基因组进化分析
Complete genomic sequence analysis of porcine circovirus type 2 from Guangxi
摘要
Abstract
To investigate the genetic variation trend of porcine circovirus type 2 (PCV2) strains of Guangxi in recent years and provide theoretical guidance for effective control of porcine circovirus disease(PCVD),lung tissues and lymph nodes of dead nursery pigs from different areas in Guangxi were tested and PCV2 complete genomes were amplified.LaserGene 7.1 and MEGA4.1 softwares were used to analyze the nucleotide and genetic evolution of PCV2 complete genomes,and the amino acid variation of the open reading frame(ORF2) was analyzed.The results showed that 12 PCV2 complete genome sequences were successfully obtained from the positive samples,in which 6 of 12 belonged to the PCV2d genotype,and others belonged to the PCV2b genotype.The deduced amino acid sequences of ORF2 from different genotype showed that the amino acid sites of ORF2 of PCV2a,PCV2b,PCV2c and PCV2d had their specificality.PCV2d genotype infectious cases in pigs increased in Guangxi regions in this study,which is likely to make PCV2d replace PCV2b or becomes a dominant genotype with PCV2b in Guangxi region.关键词
猪圆环病毒2型/全基因/ORF2/遗传进化Key words
porcine circovirus type 2/complete gene/0RF2/genetic evolution分类
农业科技引用本文复制引用
张民秀,谢芝勋,谢丽基,黄莉,谢志勤,刘加波,邓显文,罗思思,黄娇玲..广西猪圆环病毒2型全基因组进化分析[J].中国兽医科学,2017,47(4):481-488,8.基金项目
广西科技攻关重大专项(1222003-2-4) （1222003-2-4）
国家“万人计划”领军人才专项(2016-37-88) （2016-37-88）
广西特聘专家专项(2011B020) （2011B020）
