中国兽医科学2017，Vol.47Issue(4)：508-513,6.DOI:10.16656/j.issn.1673-4696.2017.04.017
小鼠诺如病毒巢式RT-PCR检测方法的建立与应用
Establishment and application of nested RT-PCR for the detection of murine norovirus
摘要
Abstract
Murine norovirus(MNV)is recognized as one of the most prevalent pathogens in laboratory mice,which causes significant effects on production of mice and scientific research.To improve and prevent this situation,establishment of a rapid and high sensitive detection method for MNVis necessary.According to a large number of genetically diverse MNVgene sequences,we designed two pairs of nested PCR primers,optimized PCR reaction conditions and detected the clinical samples.Our results suggested that the sensitivity of this method was high,the minimum detection level was 1×102 copies/μL and the detection rate of clinical samples was 73％,which was higher thanthe result of RT-PCR using one pair of referenced primers,1 ×105 copies/μ L and 60％,respectively.Briefly,the established nested PCR detection method for MNVin this study showed a powerful mean for quick and efficient detection of MNVand for estimating the prevalence of MNVinfection.关键词
小鼠诺如病毒/巢式RT-PCR/敏感性Key words
murine noroviruses/nested RT-PCR/sensitivity分类
农业科技引用本文复制引用
张旭亮,马畅,刘捷,尤金炜,刘彪,恽时锋..小鼠诺如病毒巢式RT-PCR检测方法的建立与应用[J].中国兽医科学,2017,47(4):508-513,6.基金项目
中国人民解放军南京军区南京总医院科学研究项目(2016051) （2016051）
