中国实用口腔科杂志2017，Vol.10Issue(3)：135-141,7.DOI:10.19538/j.kq.2017.03.002
组织工程颞下颌关节生物髁突裸鼠皮下再生研究
Subcutaneous regeneration of tissue-engineered mandibular condyle in nude mice
摘要
Abstract
Objective To explore the feasibility of regenerating mandibular condyles based on cartilage cell sheet with cell bone phase scaffold compared with cell-biphasic scaffolds.Methods Tissue-engineered mandibular condyles were regenerated by:1) cartilage cell sheet+bone phase scaffold (PCL/HA) seeded with bone marrow stem cells (BMSCs) from mini-pigs (cell sheet group),and 2) cartilage phase scaffold (PGA/PLA) seeded with auricular chondrocytes + bone phase scaffold seeded with BMSCs from mini-pigs (biphasic scaffold group).They were implanted subcutaneously in nude mice after being cultured in vitro for different periods of time.After 12 weeks,the nude mice were sacrificed,the specimens were harvested and evaluatedbased on gross appearance and histopathologic observations with hematoxylin and eosin,safranin O-fast green and immumohistochemical staining for collagen Ⅰ and Ⅱ.The histopathologic assessment score of condylar cartilage and bone density were compared between the 2 groups using SPSS 17.0 software.Results The two groups' specimens all formed mature cartilage-like tissues with numerous chondrocytes,typical cartilage lacuna and abundant cartilage-specific extracellular matrix.The regenerated cartilage was instant,continuous,homogeneous and avascular.In the biphasic scaffold group,there were still a few residual PGA fibers in the cartilage layer.The cartilage and bone interface was established in the 2 groups,and the microchannels of the bone phase scaffolds were filled with bone tissue.The score of cartilage regeneration in the cell sheet group was a little higher than the biphasic scaffold group,but without significant difference (P ＞ 0.05).The number of cartilage cell per unit in cell sheet group was more than that in biphasic scaffold group,but there was no statistical difference (P ＞ 0.05).There was no significant difference of bone tissue formation between the 2 groups (P ＞ 0.05).BMSCs near cartilage phase differentiated to cartilage,while those far from it differentiated to bone tissue and adipose tissue.Conclusion Both cartilage cell sheet group and biphasic scaffold group can regenerate condyle shaped osteochondral composite in nude mice.Without residual PGA fibers,the cell sheet group has less chance of immunological rejection and has wider application in the future.关键词
颞下颌关节/再生/生物髁突/裸鼠Key words
temporomandibular joint/regeneration/condyle/nude mouse分类
医药卫生引用本文复制引用
王飞宇,何冬梅,周广东,杨秀娟..组织工程颞下颌关节生物髁突裸鼠皮下再生研究[J].中国实用口腔科杂志,2017,10(3):135-141,7.基金项目
上海市科学技术委员会实验动物专项(15140902500) （15140902500）
国家自然科学基金(81472117) （81472117）
上海交通大学医工交叉基金(YG2014MS05) （YG2014MS05）
上海市科学技术委员会科研计划项目(14DZ2294300) （14DZ2294300）
