中国斜视与小儿眼科杂志2017，Vol.25Issue(1)：1-4,中插4,5.DOI:10.3969/J.ISSN.1005-328X.2017.01.001
Cdt1和Mcm6作用片段在Y79细胞中的功能研究
Analysis of Cdt1 and Mcm6 interaction domains in human retinoblastoma Y79 cells
吴星 1王凤翔 1李朝辉1
作者信息
- 1. 100853 北京,中国人民解放军总医院眼科
- 折叠
摘要
Abstract
Objective To study the effect of Cdc10 dependent transcript1 (Cdt1) and minichromosome maintenance6 (Mcm6) on cell proliferation and apoptosis in a retinoblastoma cell line (Y79),and explore the mechanism.Methods The Y79 cells were divided into five groups which group cells were transfected with different plasmids:vector plasmid、Cdt1(260-391) constructed plasmid、Cdt1(392-471) constructed plasmid、Mcm6(708-821) constructed plasmid and Mcm6 (Full length) constructed plasmid.BrdU incorporation assay and FACS analysis were performed to study the cell proliferation and apoptosis.Chromatin binding assay was used to detect the endogenous Mcm4、Mcm6 and Cdc6.Results DNA replication was inhibited and apoptosis was induced in the cells transfected with Cdt1(392-471) constructed plasmid and the cell transfected with Mcm6(708-821) constructed plasmid.The endogenous Mcm4 and Mcm6 were reduced in the two group cells.Conclusions Overexpression of Cdt1 and Mcm6 interaction domains in Y79 cells inhibit cell proliferation and induce cell apoptosis.The mechanism is probably to inhibit the DNA binding ability of MCM complex.关键词
视网膜母细胞瘤/Cdt1/Mcm6Key words
Retinoblastoma/Cdt1/Mcm6引用本文复制引用
吴星,王凤翔,李朝辉..Cdt1和Mcm6作用片段在Y79细胞中的功能研究[J].中国斜视与小儿眼科杂志,2017,25(1):1-4,中插4,5.
