中国烟草科学2017，Vol.38Issue(2)：75-80,6.DOI:10.13496/j.issn.1007-5119.2017.02.013
响应面法优化全自动索式提取仪测定烟草石油醚提取物条件
Optimization of Extraction for the Petroleum Ether Extract from Tobacco with Fully-Automatic Soxhlet Extractor by Response Surface Methodology
摘要
Abstract
In order to determine the optimal experimental parameters for the extraction of petroleum ether extract from tobacco with fully-automatic Soxhlet extractor, the single factor tests and response surface methodology (RSM) were executed to optimize the extraction parameters. The flue-cured tobacco standard material of petroleum ether extract (GBW08514a) was used. The experimental results showed that the optimal extraction parameters were as follows: boiling time/leaching time: 15/90 min, extraction temperature: 100℃, extraction solvent volume: 85 mL. Under the optimal parameters, the content of petroleum ether extract of the flue-cured tobacco standard material was 4.70% (RSD/%=1.85). Consequently the optimum parameters with fully-automatic Soxhlet extractor for the extraction of petroleum ether extract from tobacco were established by response surface methodology. The discrepancy of the results applied with the proposed method and the traditional method was negligible. Moreover this method saved the labor time and greatly improved working efficiency, which was more suitable for the rapid determination of the petroleum ether extract for a multitude of tobacco samples.关键词
烟草/石油醚提取物/响应面法/全自动索式提取仪Key words
tobacco/petroleum ether extract/response surface methodology/fully-automatic Soxhlet extractor分类
轻工纺织引用本文复制引用
林樱楠,陈旭,曹鹏云,李琼红,郭先锋,鲁世军,曹建敏..响应面法优化全自动索式提取仪测定烟草石油醚提取物条件[J].中国烟草科学,2017,38(2):75-80,6.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC06) （ASTIP-TRIC06）
公益性行业(农业)科研专项"烟草增香减害关键技术研究与示范"(201203091) （农业）
