中国药房2017，Vol.28Issue(13)：1790-1793,4.DOI:10.6039/j.issn.1001-0408.2017.13.18
神香草不同洗脱物对豚鼠离体气管平滑肌收缩的影响
Effects of the Different Elutions of Hyssopus cuspidatus on Smooth Muscle Contraction of Isolated Tracheal in Guinea Pigs
摘要
Abstract
OBJECTIVE:To investigate the effects of different elutions of Hyssopus cuspidatus on smooth muscle contraction of isolated tracheal in Guinea pigs. METHODS:Isolated tracheal rings were prepared and soaked in Krebs-Henseleit,using acetyl-choline(ACh,1×10-7 g/mL)or histamine(His,1×10-6 g/mL)to induce contraction of tracheal rings,then the effects of H. cuspi-datus water elution and 30%,50%,60%,70%,95%ethanol elutions with mass concentrations of 0.08,0.16,0.32,0.64,1.28, 2.56 mg/mL on contraction of tracheal rings were respectively investigated. Contraction curves were recorded and antispasmodic rates were calculated. Tests were treated with saline as blank control and aminophylline (0.08 mg/mL) as positive control. RE-SULTS:Compared with blank control,0.16-2.56 mg/mL 30%,50% ethanol elution,0.32-2.56 mg/mL 60% ethanol elution and 0.64-2.56 mg/mL 70%,95% ethanol elution can obviously inhibit ACh-induced contraction of tracheal rings,antispasmodic rates were obviously increased(P<0.05 or P<0.01);0.32-2.56 mg/mL water elution and 30%,50% ethanol elution,0.16-2.56 mg/mL 60%,70% ethanol elution and 1.28-2.56 mg/mL 95% ethanol elution can obviously inhibit His-induced contraction of tracheal rings,antispasmodic rates were obviously increased (P<0.05 or P<0.01). The effects of 2.56 mg/mL 60% ethanol elution ap-proach to aminophylline. CONCLUSIONS:Different elutions of H. cuspidatus has certain antagonistic effect on the ACh-induced or His-induced smooth muscle contraction of isolated tracheal in Guinea pigs;60% ethanol elution shows the strongest effect, which has similar effects with aminophylline at high mass concentration.关键词
神香草/洗脱物/离体气管/乙酰胆碱/组胺/豚鼠Key words
Hyssopus cuspidatus/Elution/Isolated tracheal/Acetylcholine/Histamine/Guinea pigs分类
医药卫生引用本文复制引用
袁凤娟,孙玉华,哈木拉提·哈斯木,王新堂,贺金华,毛艳..神香草不同洗脱物对豚鼠离体气管平滑肌收缩的影响[J].中国药房,2017,28(13):1790-1793,4.基金项目
国家自然科学基金资助项目(No.81560644) （No.81560644）
新疆维吾尔自治区中医民族医药科技人才培养项目(No.2016-03-03) （No.2016-03-03）
新疆维吾尔自治区科技计划项目(No.201417017) （No.201417017）
新疆医科大学研究生创新创业项目(No.CXCY070) （No.CXCY070）
