中国药房2017，Vol.28Issue(13)：1816-1819,4.DOI:10.6039/j.issn.1001-0408.2017.13.25
超声波-酶法提取积雪草中积雪草总苷的工艺研究
Study on the Extraction Technology of Asiaticosides from Centella asiatica by Ultrasonic-enzyme Method
摘要
Abstract
OBJECTIVE:To optimize the extraction technology of asiaticosides from Centella asiatica. METHODS:Using to-tal amounts of asiaticoside and hydroxy asiaticoside as investigation indexes,single factor and orthogonal test were used to investi-gate the enzyme amount,enzymolysis time,enzymolysis temperature,ethanol volume fraction,liquid material ratio and ultrasonic extraction time and optimize the extraction technology of asiaticosides from C. asiatica by ultrasonic-enzyme method,and verifica-tion test was conducted. RESULTS:Optimal extraction technology was as follow as cellulose dosage of 12 mg/g,10-fold liquid ma-terial ratio added into 60% ethanol,enzyme hydrolysis for 60 min at 60 ℃,ultrasonic assisted extraction for 50 min. Average ex-traction rate of total asiaticosides was 1.92%(RSD=1.83%,n=3)in verification test. CONCLUSIONS:Ultrasonic-enzyme meth-od is stable and feasible for the extraction technology of asiaticosides from C. asiatica.关键词
积雪草/积雪草苷/羟基积雪草苷/超声波/纤维素酶/提取工艺/正交试验Key words
Centella asiatica/Asiaticoside/Hydroxy asiaticoside/Ultrasonic/Cellulose/Extraction technology/Orthogonal test分类
医药卫生引用本文复制引用
张丹丹,聂绪强,张涵,赵久凤,石秀君,杨建文..超声波-酶法提取积雪草中积雪草总苷的工艺研究[J].中国药房,2017,28(13):1816-1819,4.基金项目
国家自然科学基金资助项目(No.81560712) （No.81560712）
贵州省科学技术基金项目(No.黔科合LH字〔2014〕7566号) （No.黔科合LH字〔2014〕7566号）
贵州省科技计划课题(No.黔科合重大专项字〔2015〕6010) （No.黔科合重大专项字〔2015〕6010）
