中国医科大学学报2017，Vol.46Issue(4)：294-297,4.DOI:10.12007/j.issn.0258-4646.2017.04.002
Akt和SRC在Exosomes促进同源肺癌细胞迁移中的作用
Role of Akt and SRC Pathways in Exosome-mediated Migration of Homologous Lung Adenocarninoma Cells
摘要
Abstract
Objective To investigate the effect of Exosomes derived from lung cancer cells on the migration of secretory cells and homologous tumor cells and to explore the role of PI3K/Akt and SRC signaling pathways in this process.Methods Exosomes were isolated from the supematant post density gradient centrifugation of A549,lung cancer cells.Morphology of the Exosomes was studied using transmission electron microscopy.Protein expression was analyzed using Western blotting.Cell migration was analyzed by a transwell assay.Results The double-membrane-bound Exosomes appeared as discal-shaped structures,30-100 nm in diameter.Western blotting showed that CD9 was abundant in the Exosomes.The Exosomes promoted the migration of A549 cells and their homologous tumor cells,HCC827 in a dose-dependent manner,accompanied by the activation of Akt and SRC.Conclusion The Exosomes derived from A549 (lung cancer) cells promote the migration of the secreting cells and the homologous tumor cells.The mechanism may be correlated with the activation of Akt and SRC.关键词
Exosomes/迁移/肺癌/Akt/SRCKey words
Exosomes/migration/lung cancer/Akt/SRC分类
医药卫生引用本文复制引用
解世林,胡雪君,曲晶磊,范一博,车晓芳,侯科佐,曲秀娟,刘云鹏,王晓楠,康健..Akt和SRC在Exosomes促进同源肺癌细胞迁移中的作用[J].中国医科大学学报,2017,46(4):294-297,4.基金项目
国家自然科学基金(81372546,81472193) （81372546,81472193）
辽宁省科学技术计划(2014226033,2014225013,L2014296) （2014226033,2014225013,L2014296）
辽宁省教育厅重点实验室基础研究(LZ2014037) （LZ2014037）
