中国医科大学学报2017，Vol.46Issue(4)：309-312,4.DOI:10.12007/j.issn.0258-4646.2017.04.006
人巨细胞病毒UL145相互作用蛋白的筛选和功能分析
Screening and Analysis of Proteins Interacting with HCMV UL145 from a Human Fetal Brain cDNA Library
摘要
Abstract
Objective To screen a human fetal brain cDNA library for proteins that can interact with HCMV UL145 using a yeast two-hybrid system.Methods A bait plasmid (pGBKT7-UL145) was constructed.Using HCMV UL145 as bait,a human fetal brain cDNA library was screened and proteins interacting with UL145 were identified using bioinformatic methods to sequence and analyze the positive clones.Results Three clones interacting with HCMV UL145 were found,and identified as FOXG1.Conclusion Several proteins interacting with HCMV UL145 in the human fetal brain cDNA library were identified as FOXG1,indicating that this protein may play an important role in the course of HCMV infection.关键词
人巨细胞病毒/UL145蛋白/酵母双杂交系统/人胎脑cDNA文库Key words
human cytomegalovirus/UL145 protein/yeast two-hybrid system/human fetus brain cDNA library分类
医药卫生引用本文复制引用
邹飞,王爽,吴思,孙峥嵘..人巨细胞病毒UL145相互作用蛋白的筛选和功能分析[J].中国医科大学学报,2017,46(4):309-312,4.基金项目
国家自然科学基金(81171581) （81171581）
