内科2017，Vol.12Issue(2)：150-152,180,4.DOI:10.16121/j.cnki.cn45-1347/r.2017.02.02
通过钙敏感受体调控CSE、H2S抑制肺动脉平滑肌细胞的研究
Calcium-sensing receptor suppresses pulmonary artery smooth muscle cells by CSE regulation of H2S
摘要
Abstract
Objective To investigate whether calcium-sensiing receptor (CaSR) agonist can increase the level of endogenous hydrogen sulfide (H2S) by increasing the expression of cystathionine-γ-lyase (CSE), and then inhibit the proliferation and migration of pulmonary artery smooth muscle cells.Methods Human pulmonary artery smooth muscle cells were divided into three groups: blank control group (PBS solution intervention), GdCl3 group (CaSR agonist GdCl3 intervention), NPS2390 group (CaSR inhibitor NPS2390 intervention).After incubated with drugs, the cell proliferation, cell migration and the expression of CaSR, CSE, H2S were detected, and the above indexes were compared among the three groups.Results There was significant difference in the cell proliferation, cell migration and the expression of CaSR, CSE, H2S among three groups (P<0.05), the expression of CaSR, CSE and the level of H2S in GdCl3 group were significantly higher than those in control group (P<0.05), the OD value in GdCl3 group was significantly lower than that in control group (P<0.05), the cell migration in GdCl3 group was significantly less than that in control group (P<0.05);the expression of CaSR and the level of H2S in NPS2390 group were significantly lower than those in control group (P<0.05), the OD value in NPS2390 group was significantly higher than that in control group (P<0.05), the cell migration in NPS2390 group was significantly more than that in control group (P<0.05).Conclusions CaSR agonist can increase the level of H2S by enhancing the expression of CSE, and inhibit the proliferation and migration of pulmonary artery smooth muscle cells.关键词
钙敏感受体/胱硫醚-γ-裂解酶(CSE)/硫化氢(H2S)/肺动脉平滑肌细胞Key words
Calcium-sensing receptor/Cystathionine-γ-lyase (CSE)/Hydrogen sulfide (H2S)/Pulmonary artery smooth muscle cells分类
医药卫生引用本文复制引用
梁洪华,王康,李海燕,马秀涛,黄子春,覃家锦..通过钙敏感受体调控CSE、H2S抑制肺动脉平滑肌细胞的研究[J].内科,2017,12(2):150-152,180,4.基金项目
广西自然科学基金(2013GXNSFAA019151) （2013GXNSFAA019151）
