内科2017，Vol.12Issue(2)：153-156,4.DOI:10.16121/j.cnki.cn45-1347/r.2017.02.03
将大肠杆菌OmpA信号肽改造成为抗菌肽的研究
Study on conversion of OmpA signal peptide of Escherichia coli to antimicrobial peptide
摘要
Abstract
Objective To study the method of conversing the OmpA signal peptide of E.coli into antimicrobial peptide and its bactericidal activity.Methods The OmpA signal peptide (P-AQA) of E.Coli that consists of 21 amino acids was used as template, three derived peptides(MKKTAIAIAVALAGFARVRQK,P-RQK)、(MKKTAIAIAVALAGFARRKKK,P-KKK)、(MKKTAIAIAVALAGFAD VDQD,P-DQD)were synthesized by changing the amino acid composition of the C-terminal of the OmpA signal peptide of E.coli.The bactericidal activity of the three derived peptides against gram-positive bacteria (G+) (Staphylococcus aureus) and gram-negative bacteria (G-) (Escherichia coli) was determined by agar plate counting method.Results The natural signal peptide P-AQA was insoluble in water, the bactericidal activity could not determine;the peptide P-DQD with acidic amino acid added at the C-terminal of the signal peptide had no bactericidal action on G+ and G-;the other two polypeptides with the basic amino acid at the terminal had strong bactericidal action to G+ and G-, and the bactericidal activity of the polypeptides with 5 basic amino acids at the terminal was about 5 times higher than that of 3 basic amino acids.Conclusions The derived peptide which neutral amino acid was replaced by basic amino acids in the C-terminal of the OmpA signal peptide of E.coli, with evident bactericidal activity, and the bactericidal activity might be correlated with the numbers of basic amino acids.关键词
大肠杆菌/信号肽/衍生肽/碱性氨基酸/杀菌活性Key words
Escherichia Coli/Signal peptides/Derived peptides/Basic amino acids/Bactericidal activity分类
生物科学引用本文复制引用
谭咪乐,李永杰,黄凌凌,梁宁生..将大肠杆菌OmpA信号肽改造成为抗菌肽的研究[J].内科,2017,12(2):153-156,4.基金项目
广西自然科学基金资助项目(2013GXNSFAA019250) （2013GXNSFAA019250）
广西医疗卫生重点科研课题 (重2011075) （重2011075）
