姜晖 1李开智 2王绍志 1付连浩 1张熙洁 1刘晓红1
作者信息
- 1. 河北省唐山市工人医院,河北唐山 063000
- 2. 河北省唐山市第二医院,河北唐山 063000
- 折叠
摘要
Abstract
Objective To establish an HPLC method for content determination of puerarin, polydatin, ferulic acid and emodin in Yangyin Jiangtang Tablets. Methods The Agilent Zorbax Eclipse XDB-C18 ( 150 mm × 4. 6 mm, 5 μm ) chromatographic separation column was used, mobile phase were composed of acetonitrile ( A ) -methanol ( B ) -0. 5%H3PO4 ( C ) solution in a linear gradient elution mode ( 0 min:A 10%, B 5%, C 85%;10 min:A 20%, B 5%, C 75%;20 min:A 25%, B 5%, C 70%;30 min:A 30%, B 7%, C 53%;40 min:A 50%, B 30%, C 20%;55 min:A 50%, B 30%, C 20% ) . The flow rate was 0. 6 mL/min, and the column temperature was set at 30 ℃, the injection volume was 10 μL. The detection wave length were set at 250, 306, 254, 320 nm. Results There was a good linear relationship in a range of 42. 43-678. 91 μg/mL for puerarin, 150. 00-2400. 00 μg/mL for polydatin, 0. 39-6. 26 μg/mL for ferulic acid, 62. 50-1000. 00 μg/mL for emodin, respectively. The average recovery were 98. 59%, 99. 12%, 98. 70%, 98. 89%, respec-tively, the RSDs were 0. 30%, 0. 52%, 1. 79%, 0. 61% ( n=6 ) , respectively. Conclusion The method has good separation effect, it is simple, convenient, and can control the quality of Yangyin Jiangtang Tablets.关键词
中药制剂/高效液相色谱法/含量测定/葛根素/虎杖苷/阿魏酸/大黄素Key words
preparations of traditional Chinese medicine/HPLC/content determination/puerarin/polydatin/ferulic acid/emodin分类
医药卫生
